PMID- 23911627
OWN - NLM
STAT- MEDLINE
DCOM- 20150702
LR  - 20140915
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 111
DP  - 2013 Nov 1
TI  - Influence of ionic strength and beta2-glycoprotein I concentration on 
      agglutination of like-charged phospholipid membranes.
PG  - 699-706
LID - S0927-7765(13)00428-1 [pii]
LID - 10.1016/j.colsurfb.2013.05.042 [doi]
AB  - The effect of ionic strength on adhesion between negatively charged giant 
      unilamellar vesicles induced by beta2-glycoprotein I (beta2-GPI) was studied 
      experimentally and theoretically. Measuring the effective angle of contact 
      between adhering vesicles indicated that the strength of adhesion between 
      vesicles decreases with increasing ionic strength, and increases with 
      concentration of beta2-GPI. In the theoretical part we focused on the study of the 
      average orientation of beta2-GPI near the charged membrane and its role in mediating 
      the attractive interactions between the vesicles. beta2-GPI proteins were modelled 
      as rods with internal distribution of electric charge. The predictions of Monte 
      Carlo simulations show orthogonal orientation of some of the membrane attached 
      beta2-GPI in narrow gap between two vesicles. On the contrary, at larger distances 
      between vesicles the proteins are parallelly attached to the membrane surface. A 
      local minimum of the free energy corresponding to beta2-GPI-mediated adhesion of two 
      neighbouring vesicles was predicted. The strength of adhesion was confirmed to 
      decrease at high ionic strength.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Perutkova, Sarka
AU  - Perutkova S
AD  - Laboratory of Biophysics, Faculty of Electrical Engineering, Trzaska 25, 1000 
      Ljubljana, Slovenia. Electronic address: sarka.perutkova@fe.uni-lj.si.
FAU - Frank-Bertoncelj, Mojca
AU  - Frank-Bertoncelj M
AD  - Department of Rheumatology, University Medical Centre Ljubljana, Vodnikova 62, 
      1000 Ljubljana, Slovenia.
FAU - Rozman, Blaz
AU  - Rozman B
AD  - Department of Rheumatology, University Medical Centre Ljubljana, Vodnikova 62, 
      1000 Ljubljana, Slovenia.
FAU - Kralj-Iglic, Veronika
AU  - Kralj-Iglic V
AD  - Faculty of Health Sciences, University of Ljubljana, Zdravstvena pot 5, 1000 
      Ljubljana, Slovenia.
FAU - Iglic, Ales
AU  - Iglic A
AD  - Laboratory of Biophysics, Faculty of Electrical Engineering, Trzaska 25, 1000 
      Ljubljana, Slovenia; Laboratory of Clinical Biophysics, Faculty of Medicine, 
      University of Ljubljana, Lipiceva 2, 1000 Ljubljana, Slovenia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130711
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Cardiolipins)
RN  - 0 (Phospholipids)
RN  - 0 (Unilamellar Liposomes)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Adhesiveness/drug effects
MH  - Agglutination/drug effects
MH  - Cardiolipins/chemistry
MH  - Electricity
MH  - Humans
MH  - Monte Carlo Method
MH  - Osmolar Concentration
MH  - Phospholipids/*chemistry
MH  - Thermodynamics
MH  - Unilamellar Liposomes/*chemistry
MH  - beta 2-Glycoprotein I/*pharmacology
OTO - NOTNLM
OT  - Adhesion
OT  - Beta2-glycoprotein I
OT  - Charged lipid membranes
OT  - Ionic strength
OT  - Mean field theory
OT  - Monte Carlo simulation
EDAT- 2013/08/06 06:00
MHDA- 2015/07/03 06:00
CRDT- 2013/08/06 06:00
PHST- 2013/04/25 00:00 [received]
PHST- 2013/05/28 00:00 [revised]
PHST- 2013/05/30 00:00 [accepted]
PHST- 2013/08/06 06:00 [entrez]
PHST- 2013/08/06 06:00 [pubmed]
PHST- 2015/07/03 06:00 [medline]
AID - S0927-7765(13)00428-1 [pii]
AID - 10.1016/j.colsurfb.2013.05.042 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2013 Nov 1;111:699-706. doi: 
      10.1016/j.colsurfb.2013.05.042. Epub 2013 Jul 11.