PMID- 23911654
OWN - NLM
STAT- MEDLINE
DCOM- 20141118
LR  - 20240323
IS  - 1872-9738 (Electronic)
IS  - 0892-0362 (Print)
IS  - 0892-0362 (Linking)
VI  - 39
DP  - 2013 Sep-Oct
TI  - Magnetic resonance microscopy-based analyses of the neuroanatomical effects of 
      gestational day 9 ethanol exposure in mice.
PG  - 77-83
LID - S0892-0362(13)00179-7 [pii]
LID - 10.1016/j.ntt.2013.07.009 [doi]
AB  - Animal model-based studies have shown that ethanol exposure during early 
      gestation induces developmental stage-specific abnormalities of the face and 
      brain. The exposure time-dependent variability in ethanol's teratogenic outcomes 
      is expected to contribute significantly to the wide spectrum of effects observed 
      in humans with fetal alcohol spectrum disorder (FASD). The work presented here 
      employs a mouse FASD model and magnetic resonance microscopy (MRM; high 
      resolution magnetic resonance imaging) in studies designed to further our 
      understanding of the developmental stage-specific defects of the brain that are 
      induced by ethanol. At neurulation stages, i.e. at the beginning of gestational 
      day (GD) 9 and again 4 hours later, time-mated C57Bl/6J dams were 
      intraperitoneally administered 2.9 g/kg ethanol or vehicle. Ethanol-exposed 
      fetuses were collected on GD 17, processed for MRM analysis, and results compared 
      to comparably staged controls. Linear and volume measurements as well as shape 
      changes for numerous individual brain regions were determined. GD 9 ethanol 
      exposure resulted in significantly increased septal region width, reduction of 
      cerebellar volume, and enlargement of all of the ventricles. Additionally, the 
      results of shape analyses showed that many areas of the ethanol-exposed brains 
      including the cerebral cortex, hippocampus and right striatum were significantly 
      misshapen. These data demonstrate that ethanol can induce dysmorphology that may 
      not be obvious based on volumetric analyses alone, highlight the asymmetric 
      aspects of ethanol-induced defects, and add to our understanding of ethanol's 
      developmental stage-dependent neuroteratogenesis.
CI  - (c) 2013.
FAU - Parnell, Scott E
AU  - Parnell SE
AD  - Bowles Center for Alcohol Studies, University of North Carolina, Chapel Hill, NC, 
      USA; Department of Cell Biology and Physiology, University of North Carolina, 
      Chapel Hill, NC, USA. Electronic address: sparnell@med.unc.edu.
FAU - Holloway, Hunter T
AU  - Holloway HT
FAU - O'Leary-Moore, Shonagh K
AU  - O'Leary-Moore SK
FAU - Dehart, Deborah B
AU  - Dehart DB
FAU - Paniaqua, Beatriz
AU  - Paniaqua B
FAU - Oguz, Ipek
AU  - Oguz I
FAU - Budin, Francois
AU  - Budin F
FAU - Styner, Martin A
AU  - Styner MA
FAU - Johnson, G Allan
AU  - Johnson GA
FAU - Sulik, Kathleen K
AU  - Sulik KK
LA  - eng
GR  - P41 EB015897/EB/NIBIB NIH HHS/United States
GR  - P30 HD003110/HD/NICHD NIH HHS/United States
GR  - K99 AA018697/AA/NIAAA NIH HHS/United States
GR  - K99/R00-AA018697/AA/NIAAA NIH HHS/United States
GR  - P60-AA011605/AA/NIAAA NIH HHS/United States
GR  - P30-HD03110/HD/NICHD NIH HHS/United States
GR  - U01-AA0216521/AA/NIAAA NIH HHS/United States
GR  - U01-AA017124/AA/NIAAA NIH HHS/United States
GR  - P60 AA011605/AA/NIAAA NIH HHS/United States
GR  - U54 EB005149/EB/NIBIB NIH HHS/United States
GR  - P41-EB015897/EB/NIBIB NIH HHS/United States
GR  - U01 AA017124/AA/NIAAA NIH HHS/United States
GR  - U01 AA021651/AA/NIAAA NIH HHS/United States
GR  - U54-EB005149-01/EB/NIBIB NIH HHS/United States
GR  - R00 AA018697/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130730
PL  - United States
TA  - Neurotoxicol Teratol
JT  - Neurotoxicology and teratology
JID - 8709538
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Abnormalities, Drug-Induced/*pathology
MH  - Animals
MH  - Brain/*abnormalities
MH  - Disease Models, Animal
MH  - Ethanol/*toxicity
MH  - Female
MH  - Fetal Alcohol Spectrum Disorders/*pathology
MH  - Gestational Age
MH  - Male
MH  - Mice
MH  - Neuroimaging
MH  - Pregnancy
PMC - PMC3795920
MID - NIHMS513131
OTO - NOTNLM
OT  - Brain
OT  - Ethanol
OT  - Fetal alcohol spectrum disorder
OT  - MRI
OT  - Mouse
OT  - Shape analysis
COIS- Conflict of Interest Statement No conflicts to disclose.
EDAT- 2013/08/06 06:00
MHDA- 2014/11/19 06:00
PMCR- 2014/09/01
CRDT- 2013/08/06 06:00
PHST- 2013/04/23 00:00 [received]
PHST- 2013/07/03 00:00 [revised]
PHST- 2013/07/25 00:00 [accepted]
PHST- 2013/08/06 06:00 [entrez]
PHST- 2013/08/06 06:00 [pubmed]
PHST- 2014/11/19 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - S0892-0362(13)00179-7 [pii]
AID - 10.1016/j.ntt.2013.07.009 [doi]
PST - ppublish
SO  - Neurotoxicol Teratol. 2013 Sep-Oct;39:77-83. doi: 10.1016/j.ntt.2013.07.009. Epub 
      2013 Jul 30.