PMID- 23912866
OWN - NLM
STAT- MEDLINE
DCOM- 20140130
LR  - 20130805
IS  - 1121-7138 (Print)
IS  - 1121-7138 (Linking)
VI  - 36
IP  - 3
DP  - 2013 Jul
TI  - HHV-6 is ubiquitously found using Western blot in tonsils and adenoid tissues of 
      healthy people.
PG  - 251-6
AB  - Few studies have examined the prevalence and cellular proclivity of latent human 
      herpesvirus 6 (HHV-6) in healthy populations. Difficulties in detection of HHV-6 
      genome in different tissues using polymerase chain reaction (PCR) and 
      immunohistochemistry (IHC) techniques have been reported by various researchers. 
      We examined tonsils and adenoid tissues of 54 patients who had undergone 
      tonsillectomy or adenoidectomy without any evidence of acute infection for the 
      presence of latent HHV-6 infection. While we were investigating the prevalence of 
      HHV-6, we tested the efficiency of PCR, IHC and Western Blot (WB) for detection 
      of HHV-6 in tonsil tissues. We found that 100% of tonsil tissues were positive 
      for HHV-6 with WB, 40% of tonsils were positive with PCR and no tonsil was 
      positive with IHC. This result correlates well with most studies claiming HHV-6 
      is a ubiquitous organism in various populations and tissues. Western blot may be 
      a good choice for detecting HHV-6 in tissues. Expression of the HHV-6 gp60/110 
      envelope protein disclosed by WB may indicate that HHV-6 does not have true 
      latency. To our knowledge, this is the first report to use WB to test for HHV-6 
      in tissues.
FAU - Taspinar, Mehmet
AU  - Taspinar M
AD  - Yuzuncu Yil University, School of Medicine, Department of Medical Biology, 
      Turkey.
FAU - Cetin, Nilgun
AU  - Cetin N
FAU - Gerceker, Devran
AU  - Gerceker D
FAU - Karasartova, Djursun
AU  - Karasartova D
FAU - Turegun, Buse
AU  - Turegun B
FAU - Ozturk, Sibel
AU  - Ozturk S
FAU - Sahin, Fikret
AU  - Sahin F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130630
PL  - Italy
TA  - New Microbiol
JT  - The new microbiologica
JID - 9516291
RN  - 0 (Antigens, Viral)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adenoids/*virology
MH  - Adolescent
MH  - Adult
MH  - Antigens, Viral/*immunology
MH  - Blotting, Western/*methods
MH  - Child
MH  - Child, Preschool
MH  - DNA, Viral/genetics
MH  - Female
MH  - Herpesviridae Infections/*virology
MH  - Herpesvirus 6, Human/genetics/immunology/*isolation & purification
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Organ Specificity
MH  - Palatine Tonsil/*virology
MH  - Polymerase Chain Reaction
MH  - Prevalence
MH  - Sensitivity and Specificity
MH  - Sequence Analysis, DNA
MH  - Young Adult
EDAT- 2013/08/06 06:00
MHDA- 2014/01/31 06:00
CRDT- 2013/08/06 06:00
PHST- 2012/08/06 00:00 [received]
PHST- 2012/11/12 00:00 [accepted]
PHST- 2013/08/06 06:00 [entrez]
PHST- 2013/08/06 06:00 [pubmed]
PHST- 2014/01/31 06:00 [medline]
PST - ppublish
SO  - New Microbiol. 2013 Jul;36(3):251-6. Epub 2013 Jun 30.