PMID- 23914898 OWN - NLM STAT- MEDLINE DCOM- 20131101 LR - 20211021 IS - 1471-2202 (Electronic) IS - 1471-2202 (Linking) VI - 14 DP - 2013 Aug 2 TI - OECs transplantation results in neuropathic pain associated with BDNF regulating ERK activity in rats following cord hemisection. PG - 80 LID - 10.1186/1471-2202-14-80 [doi] AB - BACKGROUND: The olfactory ensheathing cells (OECs) derived from olfactory bulb (OB) may improve motor function after transplantation in injured spinal cord. However, the effects of OEC transplantation on sensory function have not been reported yet. The purpose of this study is to investigate whether OEC transplantation could affect the sensory function and to analyze the underlying mechanism. RESULTS: OEC transplantation into the hemisected spinal cords can result in hyperalgesia, indicated by radiant and mechanical stimuli towards the plantar surface in rats. This could be associated with upregulation of Brain Derived Neurotrophic Factor (BDNF), indicated by RT-PCR. Immunofluorecent staining showed that BDNF was mainly located in the neurons of the laminas I and II of the dorsal horn. Moreover, a notable upregulation on the level of p-ERK (phosphorylation of extracellular signal-regulated kinase), the downstream molecule of BDNF, was detected by using Western Blot. These findings indicate that the increased BDNF level associated with the p-ERK was possibly involved in neuropathic pain in hemisected spinal cord subjected to OEC transplantation. CONCLUSIONS: The transplantation of OECs may induce the noticeable pain hypersensitivity in rats after hemisected spinal cord injury, and the possible mechanism may be associated with the phosphorylation of ERK and the activated BDNF overexpression. FAU - Lang, Bing-Chen AU - Lang BC AD - Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, China. FAU - Zhang, Zhuo AU - Zhang Z FAU - Lv, Long-Yun AU - Lv LY FAU - Liu, Jin AU - Liu J FAU - Wang, Ting-Yong AU - Wang TY FAU - Yang, Ling-Hui AU - Yang LH FAU - Liao, Da-Qing AU - Liao DQ FAU - Zhang, Wen-Sheng AU - Zhang WS FAU - Wang, Ting-Hua AU - Wang TH LA - eng PT - Journal Article DEP - 20130802 PL - England TA - BMC Neurosci JT - BMC neuroscience JID - 100966986 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (RNA, Messenger) RN - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/genetics/*metabolism MH - Cell Survival MH - Cell Transplantation/*adverse effects MH - Cells, Cultured MH - Disease Models, Animal MH - Extracellular Signal-Regulated MAP Kinases/genetics/*metabolism MH - Gene Expression Regulation/physiology MH - Hyperalgesia/etiology MH - Neuralgia/*etiology MH - Neuroglia/*physiology MH - Neurons/metabolism MH - Olfactory Bulb/*cytology MH - Pain Measurement MH - Pain Threshold/physiology MH - Physical Stimulation/adverse effects MH - RNA, Messenger/metabolism MH - Rats MH - Signal Transduction/physiology MH - Spinal Cord Injuries/surgery PMC - PMC3734147 EDAT- 2013/08/07 06:00 MHDA- 2013/11/02 06:00 PMCR- 2013/08/02 CRDT- 2013/08/07 06:00 PHST- 2012/10/11 00:00 [received] PHST- 2013/07/11 00:00 [accepted] PHST- 2013/08/07 06:00 [entrez] PHST- 2013/08/07 06:00 [pubmed] PHST- 2013/11/02 06:00 [medline] PHST- 2013/08/02 00:00 [pmc-release] AID - 1471-2202-14-80 [pii] AID - 10.1186/1471-2202-14-80 [doi] PST - epublish SO - BMC Neurosci. 2013 Aug 2;14:80. doi: 10.1186/1471-2202-14-80.