PMID- 23916509
OWN - NLM
STAT- MEDLINE
DCOM- 20140515
LR  - 20210702
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 552
DP  - 2013 Sep 27
TI  - Functional expression of TRPV1 and TRPA1 in rat vestibular ganglia.
PG  - 92-7
LID - S0304-3940(13)00665-4 [pii]
LID - 10.1016/j.neulet.2013.07.019 [doi]
AB  - Both TRPV1 and TRPA1 are non-selective cation channels. They are co-expressed, 
      and interact in sensory neurons such as dorsal root ganglia (DRG) and trigeminal 
      ganglia (TG), and are involved in nociception, being activated by nociceptive 
      stimuli. Immunohistological localization of TRPV1 in vestibular ganglion (VG) 
      neurons has been reported. Although TRPA1 is co-expressed with TRPV1 in DRG and 
      TG neurons, it is unclear whether TRPA1 channels are expressed in VG neurons. 
      Moreover, it is unknown whether TRPV1 and TRPA1 channels are functional in VG 
      neurons. We investigated the expression of TRPV1 and TRPA1 in rat VG neurons by 
      RT-PCR, in situ hybridization, immunohistochemistry, and Ca(2+) imaging 
      experiments. Both TRPV1 and TRPA1 RT-PCR products were amplified from the mRNA of 
      rat VG neurons. In situ hybridization experiments showed TRPV1 and TRPA1 mRNA 
      expression in the majority of VG neurons. Immunohistochemistry experiments 
      confirmed TRPV1 protein expression. In Ca(2+) imaging experiments, capsaicin, a 
      TRPV1 agonist, induced a significant increase in intracellular calcium ion 
      concentration ([Ca(2+)]i) in rat primary cultured VG neurons, which was almost 
      completely blocked by capsazepine, a TRPV1-specific antagonist. Cinnamaldehyde, a 
      TRPA1 agonist, also caused an increase in [Ca(2+)]i, which was completely 
      inhibited by HC030031, a TRPA1-specific antagonist. Moreover, in some VG neurons, 
      a [Ca(2+)]i increase was evoked by both capsaicin and cinnamaldehyde in the same 
      neuron. In summary, our histological and physiological studies reveal that TRPV1 
      and TRPA1 are expressed in VG neurons. It is suggested that TRPV1 and TRPA1 in VG 
      neurons might participate in vestibular function and/or dysfunction such as 
      vertigo.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Kamakura, Takefumi
AU  - Kamakura T
AD  - Department of Otorhinolaryngology - Head and Neck Surgery, Osaka University 
      Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan; 
      Department of Neuroscience and Cell Biology, Osaka University Graduate School of 
      Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
FAU - Ishida, Yusuke
AU  - Ishida Y
FAU - Nakamura, Yukiko
AU  - Nakamura Y
FAU - Yamada, Takahiro
AU  - Yamada T
FAU - Kitahara, Tadashi
AU  - Kitahara T
FAU - Takimoto, Yasumitsu
AU  - Takimoto Y
FAU - Horii, Arata
AU  - Horii A
FAU - Uno, Atsuhiko
AU  - Uno A
FAU - Imai, Takao
AU  - Imai T
FAU - Okazaki, Suzuyo
AU  - Okazaki S
FAU - Inohara, Hidenori
AU  - Inohara H
FAU - Shimada, Shoichi
AU  - Shimada S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130803
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 
      (2-(1,3-dimethyl-2,6-dioxo-1,2,3,6-tetrahydro-7H-purin-7-yl)-N-(4-isopropylphenyl)acetamide)
RN  - 0 (Acetanilides)
RN  - 0 (Purines)
RN  - 0 (TRPA1 Cation Channel)
RN  - 0 (TRPC Cation Channels)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (Trpa1 protein, rat)
RN  - 0 (Trpv1 protein, rat)
RN  - 7864XYD3JJ (Acrolein)
RN  - LFW48MY844 (capsazepine)
RN  - S07O44R1ZM (Capsaicin)
RN  - SR60A3XG0F (cinnamaldehyde)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Acetanilides/pharmacology
MH  - Acrolein/analogs & derivatives/pharmacology
MH  - Animals
MH  - Calcium/metabolism
MH  - Capsaicin/analogs & derivatives/pharmacology
MH  - Molecular Imaging
MH  - Primary Cell Culture
MH  - Purines/pharmacology
MH  - Rats
MH  - TRPA1 Cation Channel
MH  - TRPC Cation Channels/agonists/antagonists & inhibitors/biosynthesis/*physiology
MH  - TRPV Cation Channels/agonists/antagonists & inhibitors/biosynthesis/*physiology
MH  - Vestibular Nerve/drug effects/*metabolism
OTO - NOTNLM
OT  - Ca(2+) imaging experiment
OT  - In situ hybridization
OT  - TRPA1
OT  - TRPV1
OT  - Vestibular ganglia
EDAT- 2013/08/07 06:00
MHDA- 2014/05/16 06:00
CRDT- 2013/08/07 06:00
PHST- 2012/12/10 00:00 [received]
PHST- 2013/06/15 00:00 [revised]
PHST- 2013/07/15 00:00 [accepted]
PHST- 2013/08/07 06:00 [entrez]
PHST- 2013/08/07 06:00 [pubmed]
PHST- 2014/05/16 06:00 [medline]
AID - S0304-3940(13)00665-4 [pii]
AID - 10.1016/j.neulet.2013.07.019 [doi]
PST - ppublish
SO  - Neurosci Lett. 2013 Sep 27;552:92-7. doi: 10.1016/j.neulet.2013.07.019. Epub 2013 
      Aug 3.