PMID- 23918351
OWN - NLM
STAT- MEDLINE
DCOM- 20140528
LR  - 20130916
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 91
IP  - 10
DP  - 2013 Oct
TI  - Brain-derived neurotrophic factor is upregulated in the cervical dorsal root 
      ganglia and spinal cord and contributes to the maintenance of pain from facet 
      joint injury in the rat.
PG  - 1312-21
LID - 10.1002/jnr.23254 [doi]
AB  - The facet joint is commonly associated with neck and low back pain and is 
      susceptible to loading-induced injury. Although tensile loading of the cervical 
      facet joint has been associated with inflammation and neuronal hyperexcitability, 
      the mechanisms of joint loading-induced pain remain unknown. Altered 
      brain-derived neurotrophic factor (BDNF) levels are associated with a host of 
      painful conditions, but the role of BDNF in loading-induced joint pain remains 
      undefined. Separate groups of rats underwent a painful cervical facet joint 
      distraction or a sham procedure. Bilateral forepaw mechanical hypersensitivity 
      was assessed and BDNF mRNA and protein levels were quantified in the dorsal root 
      ganglion (DRG) and spinal cord at days 1 and 7. Facet joint distraction induced 
      significant (P < 0.001) mechanical hypersensitivity at both time points. Painful 
      joint distraction did not alter BDNF mRNA in the DRG compared with sham levels 
      but did significantly increase (P < 0.016) BDNF protein expression over sham in 
      the DRG at day 7. Painful distraction also significantly increased BDNF mRNA 
      (P = 0.031) and protein expression (P = 0.047) over sham responses in the spinal 
      cord at day 7. In a separate study, intrathecal administration of the 
      BDNF-sequestering molecule trkB-Fc on day 5 after injury partially attenuated 
      behavioral sensitivity after joint distraction and reduced pERK in the spinal 
      cord at day 7 (P < 0.045). Changes in BDNF after painful facet joint injury and 
      the effect of spinal BDNF sequestration in partially reducing pain suggest that 
      BDNF signaling contributes to the maintenance of loading-induced facet pain but 
      that additional cellular responses are also likely involved.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Kras, Jeffrey V
AU  - Kras JV
AD  - Department of Bioengineering, University of Pennsylvania, Philadelphia, 
      Pennsylvania.
FAU - Weisshaar, Christine L
AU  - Weisshaar CL
FAU - Quindlen, Julia
AU  - Quindlen J
FAU - Winkelstein, Beth A
AU  - Winkelstein BA
LA  - eng
GR  - AR056288/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130806
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cervical Vertebrae
MH  - Disease Models, Animal
MH  - Ganglia, Spinal/*metabolism
MH  - Hyperalgesia/metabolism/physiopathology
MH  - Male
MH  - Pain/*metabolism/physiopathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/physiology
MH  - Spinal Cord/*metabolism
MH  - Up-Regulation
MH  - Zygapophyseal Joint/*injuries
OTO - NOTNLM
OT  - BDNF
OT  - ERK
OT  - facet joint
OT  - pain
OT  - trkB
EDAT- 2013/08/07 06:00
MHDA- 2014/05/29 06:00
CRDT- 2013/08/07 06:00
PHST- 2013/02/19 00:00 [received]
PHST- 2013/04/26 00:00 [revised]
PHST- 2013/04/30 00:00 [accepted]
PHST- 2013/08/07 06:00 [entrez]
PHST- 2013/08/07 06:00 [pubmed]
PHST- 2014/05/29 06:00 [medline]
AID - 10.1002/jnr.23254 [doi]
PST - ppublish
SO  - J Neurosci Res. 2013 Oct;91(10):1312-21. doi: 10.1002/jnr.23254. Epub 2013 Aug 6.