PMID- 23918967
OWN - NLM
STAT- MEDLINE
DCOM- 20131022
LR  - 20240109
IS  - 1460-2431 (Electronic)
IS  - 0022-0957 (Linking)
VI  - 64
IP  - 11
DP  - 2013 Aug
TI  - Auxin induces redox regulation of ascorbate peroxidase 1 activity by 
      S-nitrosylation/denitrosylation balance resulting in changes of root growth 
      pattern in Arabidopsis.
PG  - 3339-49
LID - 10.1093/jxb/ert172 [doi]
AB  - S-Nitrosylation of Cys residues is one of the molecular mechanisms driven by 
      nitric oxide (NO) for regulating biological functions of key proteins. While the 
      studies on S-nitrosylation of Cys residues have served for identifying SNO 
      proteomes, the physiological relevance of protein S-nitrosylation/denitrosylation 
      remains poorly understood. In this study, it is shown that auxin influences the 
      balance of S-nitrosylated/denitrosylated proteins in roots of Arabidopsis 
      seedlings. 2D-PAGE allowed the identification of ascorbate peroxidase 1 (APX1) as 
      target of auxin-induced denitrosylation in roots. Auxin causes APX1 
      denitrosylation and partial inhibition of APX1 activity in Arabidopsis roots. In 
      agreement, the S-nitrosylated form of recombinant APX1 expressed in Escherichia 
      coli is more active than the denitrosylated form. Consistently, Arabidopsis apx1 
      mutants have increased H(2)O(2) accumulation in roots, shorter roots, and less 
      sensitivity to auxin than the wild type. It is postulated that an auxin-regulated 
      counterbalance of APX1 S-nitrosylation/denitrosylation contributes to a 
      fine-tuned control of root development and determination of root architecture.
FAU - Correa-Aragunde, Natalia
AU  - Correa-Aragunde N
AD  - Instituto de Investigaciones Biologicas, Facultad de Ciencias Exactas y 
      Naturales, Universidad Nacional de Mar del Plata. CC 1245, 7600 Mar del Plata, 
      Argentina.
FAU - Foresi, Noelia
AU  - Foresi N
FAU - Delledonne, Massimo
AU  - Delledonne M
FAU - Lamattina, Lorenzo
AU  - Lamattina L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Exp Bot
JT  - Journal of experimental botany
JID - 9882906
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Indoleacetic Acids)
RN  - EC 1.11.1.11 (Ascorbate Peroxidases)
SB  - IM
MH  - Arabidopsis/drug effects/*metabolism
MH  - Arabidopsis Proteins/*metabolism
MH  - Ascorbate Peroxidases/*metabolism
MH  - Enzyme Activation/drug effects
MH  - Indoleacetic Acids/*pharmacology
MH  - Oxidation-Reduction/*drug effects
MH  - Plant Roots/drug effects/*metabolism
OTO - NOTNLM
OT  - Arabidopsis
OT  - S-nitrosylation
OT  - ascorbate peroxidase 1
OT  - auxin
OT  - nitric oxide
OT  - root.
EDAT- 2013/08/07 06:00
MHDA- 2013/10/23 06:00
CRDT- 2013/08/07 06:00
PHST- 2013/08/07 06:00 [entrez]
PHST- 2013/08/07 06:00 [pubmed]
PHST- 2013/10/23 06:00 [medline]
AID - ert172 [pii]
AID - 10.1093/jxb/ert172 [doi]
PST - ppublish
SO  - J Exp Bot. 2013 Aug;64(11):3339-49. doi: 10.1093/jxb/ert172.