PMID- 23919258
OWN - NLM
STAT- MEDLINE
DCOM- 20140904
LR  - 20140116
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 49
IP  - 1
DP  - 2014 Feb
TI  - Short-term storage and swim-up selection do not affect the x/y ratio in equine 
      spermatozoa.
PG  - 52-8
LID - 10.1111/rda.12223 [doi]
AB  - The standard procedure of artificial insemination with fresh equine spermatozoa 
      involves short-term storage (to 48 h at 5 degrees C). This procedure is accompanied by a 
      gradual loss of sperm viability. The aim of this study was to investigate whether 
      the X/Y ratio of equine spermatozoa is affected by short-term storage and the 
      swim-up procedure. We used a standard protocol, for short-term storage (0, 24 and 
      48 h at 5 degrees C) of stallion semen diluted in the commercial extender EquiPro 
      (Minitub GmbH, Tiefenbach, Germany). After each set-up storage period, the motile 
      fraction of sperm cells was selected by the swim-up method. The X/Y ratio was 
      evaluated by fluorescence in situ hybridization (FISH) in the fresh, non-selected 
      sperm, and in motile spermatozoa selected after each of the storage periods. 
      Molecular probes for the equine chromosomes X and Y were used. The X/Y ratio in 
      all sperm samples analysed in this study (fresh and stored) was not different 
      from the theoretical 1 : 1 value. The incidence of chromosomally abnormal sperm 
      cells in the fresh (0.28%) and motile (0.13%) sperm samples was not significantly 
      different. The two approaches (sperm storage up to 48 h and the swim-up 
      procedure) applied to this study did not affect the X/Y ratio in the motile 
      fraction of equine spermatozoa. This finding does not conform to phenomena 
      described for human and cattle. For this reason, the finding may imply 
      species-related differences.
CI  - (c) 2013 Blackwell Verlag GmbH.
FAU - Orsztynowicz, M
AU  - Orsztynowicz M
AD  - Department of Genetics and Animal Breeding, Poznan University of Life Sciences, 
      Poznan, Poland.
FAU - Pawlak, P
AU  - Pawlak P
FAU - Kociucka, B
AU  - Kociucka B
FAU - Mucha, S
AU  - Mucha S
FAU - Klukowska-Rotzler, J
AU  - Klukowska-Rotzler J
FAU - Lechniak, D
AU  - Lechniak D
LA  - eng
PT  - Journal Article
DEP - 20130807
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
SB  - IM
MH  - Animals
MH  - Cell Separation/methods/*veterinary
MH  - *Horses
MH  - In Situ Hybridization, Fluorescence
MH  - Insemination, Artificial/methods/veterinary
MH  - Male
MH  - Semen Preservation/methods/*veterinary
MH  - Sperm Count/veterinary
MH  - Sperm Motility
MH  - Spermatozoa/*cytology
EDAT- 2013/08/08 06:00
MHDA- 2014/09/05 06:00
CRDT- 2013/08/08 06:00
PHST- 2013/04/02 00:00 [received]
PHST- 2013/06/26 00:00 [accepted]
PHST- 2013/08/08 06:00 [entrez]
PHST- 2013/08/08 06:00 [pubmed]
PHST- 2014/09/05 06:00 [medline]
AID - 10.1111/rda.12223 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2014 Feb;49(1):52-8. doi: 10.1111/rda.12223. Epub 2013 Aug 7.