PMID- 23919280
OWN - NLM
STAT- MEDLINE
DCOM- 20140422
LR  - 20130917
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 85
IP  - 18
DP  - 2013 Sep 17
TI  - Magnetite nanoparticle-induced fluorescence quenching of adenosine 
      triphosphate-BODIPY Conjugates: application to adenosine triphosphate and 
      pyrophosphate sensing.
PG  - 8559-65
LID - 10.1021/ac400919j [doi]
AB  - We report that magnetite nanoparticles (Fe3O4 NPs) act as an efficient quencher 
      for boron dipyrromethene-conjugated adenosine 5'-triphosphate (BODIPY-ATP) that 
      is highly fluorescent in bulk solution. BODIPY-ATP molecules attached to the 
      surface of Fe3O4 NPs through the coordination between the triphosphate group of 
      BODIPY-ATP and Fe(3+)/Fe(2+) on the NP surface. The formed complexes induced an 
      apparent reduction in the BODIPY-ATP fluorescence resulting from an 
      oxidative-photoinduced electron transfer (PET) from the BODIPY-ATP excited state 
      to an unfilled d shell of Fe(3+)/Fe(2+) on the NP surface. A comparison of the 
      Stern-Volmer quenching constant between Fe(3+) and Fe(2+) suggests that Fe(3+) on 
      the NP surface dominantly controls this quenching process. The efficiency for 
      Fe3O4 NP-induced fluorescence quenching of the BODIPY-ATP was enhanced by 
      increasing the concentration of Fe3O4 NPs and lowering the pH of the solution to 
      below 6.0. We found that pyrophosphate and ATP compete with BODIPY-ATP for 
      binding to Fe3O4 NPs. Thus, we amplified BODIPY-ATP fluorescence in the presence 
      of increasing the pyrophosphate and ATP concentration; the detection limits at a 
      signal-to-noise ratio of 3 for pyrophosphate and ATP were determined to be 7 and 
      30 nM, respectively. The Fe3O4 NP-based competitive binding assay detected ATP 
      and pyrophosphate in only 5 min. The selectivity of this assay for ATP over metal 
      ions, amino acids, and adenosine analogues is particularly high. The practicality 
      of using the developed method to determine ATP in a single drop of blood is also 
      validated.
FAU - Yu, Cheng-Ju
AU  - Yu CJ
AD  - Department of Chemistry, National Sun Yat-sen University , Taiwan.
FAU - Wu, Su-Mei
AU  - Wu SM
FAU - Tseng, Wei-Lung
AU  - Tseng WL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130903
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)
RN  - 0 (Boron Compounds)
RN  - 0 (Diphosphates)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Magnetite Nanoparticles)
RN  - 4E862E7GRQ (diphosphoric acid)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/*analysis/blood
MH  - Biosensing Techniques/*methods
MH  - Boron Compounds/*chemistry
MH  - Diphosphates/*analysis
MH  - Fluorescent Dyes/*chemistry
MH  - Humans
MH  - Magnetite Nanoparticles/*chemistry
MH  - Spectrometry, Fluorescence/methods
EDAT- 2013/08/08 06:00
MHDA- 2014/04/23 06:00
CRDT- 2013/08/08 06:00
PHST- 2013/08/08 06:00 [entrez]
PHST- 2013/08/08 06:00 [pubmed]
PHST- 2014/04/23 06:00 [medline]
AID - 10.1021/ac400919j [doi]
PST - ppublish
SO  - Anal Chem. 2013 Sep 17;85(18):8559-65. doi: 10.1021/ac400919j. Epub 2013 Sep 3.