PMID- 23920241
OWN - NLM
STAT- MEDLINE
DCOM- 20131127
LR  - 20220321
IS  - 1090-2430 (Electronic)
IS  - 0014-4886 (Linking)
VI  - 248
DP  - 2013 Oct
TI  - Caffeine and modafinil promote adult neuronal cell proliferation during 48 h of 
      total sleep deprivation in rat dentate gyrus.
PG  - 470-81
LID - S0014-4886(13)00238-0 [pii]
LID - 10.1016/j.expneurol.2013.07.021 [doi]
AB  - It has been established that sleep deprivation (SD) reduces the proliferation of 
      neuronal precursors in the adult hippocampus. It has also been reported that 
      psychostimulant drugs modulate adult neurogenesis. We examined the modulatory 
      role of two psychostimulant drugs modafinil and caffeine on adult neuronal cell 
      proliferation (NCP) during 48 h of total SD. A novel automated cage shaking 
      stimulus was used to induce SD based on animal activity. 5-Bromo-2''-deoxyuridine 
      (BrdU; 50mg/kg/day i.p.) was injected at the onset of the light phase for two 
      days. Rats were successfully sleep deprived for 85-94% of total time. 
      Stereological analysis showed that both caffeine and modafinil treatments during 
      SD improved the number of BrdU positive cells as compared to the SD group. 
      Caffeine treatment during SD, significantly increased early proliferative and 
      post-mitotic stages of doublecortin (DCX) positive cells while modafinil 
      treatment during SD, increased intermediate and post-mitotic stages of DCX 
      positive cells compared to SD+Vehicle group. Brain-Derived Neurotrophic Factor 
      (BDNF) expression on BrdU positive cells as well as in the dentate gyrus (DG) 
      region was decreased significantly after sleep deprivation. Both caffeine and 
      modafinil significantly improved BDNF expression in the DG region. Modafinil, but 
      not caffeine, significantly decreased hippocampal adenosine level during SD in 
      comparison to the SD+Vehicle group. It may be concluded that caffeine or 
      modafinil treatment during 48 h of SD prevents the SD induced decline in neuronal 
      proliferation and differentiation. Caffeine and modafinil induced alterations of 
      NCP during SD may involve modulation of BDNF and adenosine levels.
CI  - (c) 2013.
FAU - Sahu, Surajit
AU  - Sahu S
AD  - Neurophysiology Division, Defense Institute of Physiology and Allied Sciences 
      (DIPAS), Defense Research and Development Organization (DRDO), Lucknow Road, 
      Timarpur, Delhi-54, India.
FAU - Kauser, Hina
AU  - Kauser H
FAU - Ray, Koushik
AU  - Ray K
FAU - Kishore, Krishna
AU  - Kishore K
FAU - Kumar, Sanjeev
AU  - Kumar S
FAU - Panjwani, Usha
AU  - Panjwani U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130803
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Benzhydryl Compounds)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Central Nervous System Stimulants)
RN  - 0 (Dcx protein, mouse)
RN  - 0 (Dcx protein, rat)
RN  - 0 (Doublecortin Protein)
RN  - 3G6A5W338E (Caffeine)
RN  - R3UK8X3U3D (Modafinil)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Animals
MH  - Benzhydryl Compounds/*pharmacology
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Caffeine/*pharmacology
MH  - Cell Count
MH  - Cell Proliferation/*drug effects
MH  - Central Nervous System Stimulants/*pharmacology
MH  - Corticosterone/blood
MH  - Dentate Gyrus/cytology/*drug effects/metabolism
MH  - Doublecortin Protein
MH  - Male
MH  - Modafinil
MH  - Neurons/*drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sleep Deprivation/*metabolism
OTO - NOTNLM
OT  - 3,3'-diaminobenzidine
OT  - AC
OT  - ANOVA
OT  - Ad A1
OT  - Adenosine
OT  - Adult neuronal cell proliferation
OT  - BDNF
OT  - Brain-Derived Neurotrophic Factor
OT  - BrdU
OT  - CC
OT  - Caffeine
OT  - DAB
OT  - DCX
OT  - DG
OT  - EEG
OT  - EMG
OT  - GFAP
OT  - Gt
OT  - HC
OT  - HPLC
OT  - HRP
OT  - Modafinil
OT  - Ms
OT  - NCP
OT  - NREM
OT  - PBS
OT  - PDE
OT  - PFA
OT  - REM
OT  - RT
OT  - Rb
OT  - Rt
OT  - SD
OT  - SD+CAF
OT  - SD+MDF
OT  - SDS
OT  - SEM
OT  - Sleep deprivation
OT  - adenosine A1 receptor
OT  - adult neuronal cell proliferation
OT  - analysis of variance
OT  - apparatus control
OT  - bromodeoxyurdine
OT  - cage control
OT  - dentate gyrus
OT  - doublecortin
OT  - electroencephalogram
OT  - electromyogram
OT  - glial fibrillary acidic protein
OT  - goat
OT  - high pressure liquid chromatography
OT  - hippocampus
OT  - horseradish peroxidase
OT  - mouse
OT  - non-rapid eye movement
OT  - paraformaldehyde in PBS
OT  - phosphate buffered saline
OT  - phosphodiesterase
OT  - rabbit
OT  - rapid eye movement
OT  - rat
OT  - room temperature
OT  - sleep deprivation
OT  - sleep deprivation syndrome
OT  - sleep deprived for 48h and caffeine treated
OT  - sleep deprived for 48h and modafinil treated
OT  - sleep deprived for 48h and saline treated
OT  - standard error of mean
EDAT- 2013/08/08 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/08/08 06:00
PHST- 2013/03/31 00:00 [received]
PHST- 2013/07/15 00:00 [revised]
PHST- 2013/07/29 00:00 [accepted]
PHST- 2013/08/08 06:00 [entrez]
PHST- 2013/08/08 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - S0014-4886(13)00238-0 [pii]
AID - 10.1016/j.expneurol.2013.07.021 [doi]
PST - ppublish
SO  - Exp Neurol. 2013 Oct;248:470-81. doi: 10.1016/j.expneurol.2013.07.021. Epub 2013 
      Aug 3.