PMID- 23921963
OWN - NLM
STAT- MEDLINE
DCOM- 20140602
LR  - 20211021
IS  - 1536-4828 (Electronic)
IS  - 0885-3177 (Print)
IS  - 0885-3177 (Linking)
VI  - 42
IP  - 7
DP  - 2013 Oct
TI  - Expression of MUC4 mucin is observed mainly in the intestinal type of intraductal 
      papillary mucinous neoplasm of the pancreas.
PG  - 1120-8
LID - 10.1097/MPA.0b013e3182965915 [doi]
AB  - OBJECTIVES: This study aimed to examine expression profile of MUC4 in intraductal 
      papillary mucinous neoplasm of the pancreas (IPMN). METHODS: We performed 
      immunohistochemistry (IHC) of MUC4 in 142 IPMNs, with evaluation of the 
      specificity of 2 anti-MUC4 monoclonal antibodies, 8G7 and 1G8, in cancer cell 
      lines. RESULTS: Monoclonal antibody 8G7 showed a clear immunoreactivity, whereas 
      MAb 1G8 did not show any immunoreactivity, in the Western blotting and IHC for 
      human pancreatic carcinoma cell lines expressing MUC4 messenger RNA. However, IHC 
      signals detected by both monoclonal antibodies were observed in the tissue 
      specimens. The expression rates of MUC4/8G7 detected by MAb 8G7 and MUC4/1G8 
      detected by MAb 1G8 in the intestinal-type IPMNs were significantly higher than 
      those in the gastric-type IPMNs. In the intestinal-type IPMNs, MUC4/8G7 was 
      expressed mainly in the cytoplasm of the neoplastic cells, whereas MUC4/1G8 was 
      expressed mainly at the cell apexes. Even in the gastric-type IPMNs with rare 
      MUC4 expression in the low-grade dysplasia, both MUC4 expression rates increased 
      when dysplasia advanced. CONCLUSIONS: A significantly higher expression of MUC4 
      in intestinal-type IPMNs than in gastric-type IPMNs will be one of the biomarkers 
      to discriminate between the intestinal-type IPMNs with high malignancy potential 
      from gastric-type IPMNs with low malignancy potential.
FAU - Kitazono, Iwao
AU  - Kitazono I
AD  - From the *Department of Human Pathology, Field of Oncology, and daggerCardiovascular 
      and Gastroenterological Surgery, Graduate School of Medical and Dental Sciences, 
      Kagoshima University; double daggerDepartment of Pathology, and  section signDepartment of Surgery, 
      Kagoshima-shi Medical Association Hospital, Kagoshima, Japan;  parallelDepartments of 
      Biochemistry and Molecular Biology, Eppley Institute for Research in Cancer and 
      Allied Diseases, University of Nebraska Medical Center, Omaha, NE; and  paragraph signNational 
      Sanatorium Hoshizuka-Keiaien, Kanoya, Kagoshima, Japan.
FAU - Higashi, Michiyo
AU  - Higashi M
FAU - Kitamoto, Sho
AU  - Kitamoto S
FAU - Yokoyama, Seiya
AU  - Yokoyama S
FAU - Horinouchi, Michiko
AU  - Horinouchi M
FAU - Osako, Masahiko
AU  - Osako M
FAU - Shimizu, Takeshi
AU  - Shimizu T
FAU - Tabata, Mineo
AU  - Tabata M
FAU - Batra, Surinder K
AU  - Batra SK
FAU - Goto, Masamichi
AU  - Goto M
FAU - Yonezawa, Suguru
AU  - Yonezawa S
LA  - eng
GR  - R03 CA133944/CA/NCI NIH HHS/United States
GR  - U54 CA163120/CA/NCI NIH HHS/United States
GR  - U01 CA111294/CA/NCI NIH HHS/United States
GR  - R01 CA078590/CA/NCI NIH HHS/United States
GR  - CA133944/CA/NCI NIH HHS/United States
GR  - CA163120/CA/NCI NIH HHS/United States
GR  - CA111294/CA/NCI NIH HHS/United States
GR  - R01 CA131944/CA/NCI NIH HHS/United States
GR  - CA78590/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Pancreas
JT  - Pancreas
JID - 8608542
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (MUC4 protein, human)
RN  - 0 (Mucin-4)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Antibody Specificity
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Cystadenoma, Mucinous/genetics/*metabolism/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - Mucin-4/genetics/*metabolism
MH  - Pancreatic Neoplasms/genetics/*metabolism/pathology
MH  - RNA, Messenger/genetics/metabolism
MH  - RNA, Neoplasm/genetics/metabolism
MH  - Transcriptome
PMC - PMC3779537
MID - NIHMS478866
EDAT- 2013/08/08 06:00
MHDA- 2014/06/03 06:00
PMCR- 2014/10/01
CRDT- 2013/08/08 06:00
PHST- 2013/08/08 06:00 [entrez]
PHST- 2013/08/08 06:00 [pubmed]
PHST- 2014/06/03 06:00 [medline]
PHST- 2014/10/01 00:00 [pmc-release]
AID - 10.1097/MPA.0b013e3182965915 [doi]
PST - ppublish
SO  - Pancreas. 2013 Oct;42(7):1120-8. doi: 10.1097/MPA.0b013e3182965915.