PMID- 23922760
OWN - NLM
STAT- MEDLINE
DCOM- 20140429
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 7
DP  - 2013
TI  - Transcriptomic analysis of insulin-sensitive tissues from anti-diabetic drug 
      treated ZDF rats, a T2DM animal model.
PG  - e69624
LID - 10.1371/journal.pone.0069624 [doi]
LID - e69624
AB  - Gene expression changes have been associated with type 2 diabetes mellitus 
      (T2DM); however, the alterations are not fully understood. We investigated the 
      effects of anti-diabetic drugs on gene expression in Zucker diabetic fatty (ZDF) 
      rats using oligonucleotide microarray technology to identify gene expression 
      changes occurring in T2DM. Global gene expression in the pancreas, adipose 
      tissue, skeletal muscle, and liver was profiled from Zucker lean control (ZLC) 
      and anti-diabetic drug treated ZDF rats compared with those in ZDF rats. We 
      showed that anti-diabetic drugs regulate the expression of a large number of 
      genes. We provided a more integrated view of the diabetic changes by examining 
      the gene expression networks. The resulting sub-networks allowed us to identify 
      several biological processes that were significantly enriched by the 
      anti-diabetic drug treatment, including oxidative phosphorylation (OXPHOS), 
      systemic lupus erythematous, and the chemokine signaling pathway. Among them, we 
      found that white adipose tissue from ZDF rats showed decreased expression of a 
      set of OXPHOS genes that were normalized by rosiglitazone treatment accompanied 
      by rescued blood glucose levels. In conclusion, we suggest that alterations in 
      OXPHOS gene expression in white adipose tissue may play a role in the 
      pathogenesis and drug mediated recovery of T2DM through a comprehensive gene 
      expression network study after multi-drug treatment of ZDF rats.
FAU - Kim, Yo Na
AU  - Kim YN
AD  - Laboratory of Developmental Biology and Genomics, College of Veterinary Medicine, 
      Research Institute for Veterinary Science, BK21 Program for Veterinary Science, 
      Seoul National University, Seoul, Korea.
FAU - Kim, Sangok
AU  - Kim S
FAU - Kim, Il-Yong
AU  - Kim IY
FAU - Shin, Jae Hoon
AU  - Shin JH
FAU - Cho, Sooyoung
AU  - Cho S
FAU - Yi, Sun Shin
AU  - Yi SS
FAU - Kim, Wan Kyu
AU  - Kim WK
FAU - Kim, Kyung-Sub
AU  - Kim KS
FAU - Lee, Sanghyuk
AU  - Lee S
FAU - Seong, Je Kyung
AU  - Seong JK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130726
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
SB  - IM
MH  - Animals
MH  - Cluster Analysis
MH  - Diabetes Mellitus, Experimental/drug therapy/*genetics
MH  - Diabetes Mellitus, Type 2/*drug therapy/*genetics
MH  - Disease Models, Animal
MH  - *Gene Expression Profiling
MH  - Gene Regulatory Networks/drug effects
MH  - Glucose Tolerance Test
MH  - Hypoglycemic Agents/*pharmacology/therapeutic use
MH  - Insulin/*pharmacology
MH  - Male
MH  - Organ Specificity/drug effects/*genetics
MH  - Oxidative Phosphorylation/drug effects
MH  - Rats
MH  - Rats, Zucker
MH  - Real-Time Polymerase Chain Reaction
MH  - Reproducibility of Results
MH  - Transcription, Genetic/drug effects
PMC - PMC3724940
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/08/08 06:00
MHDA- 2014/04/30 06:00
PMCR- 2013/07/26
CRDT- 2013/08/08 06:00
PHST- 2013/04/12 00:00 [received]
PHST- 2013/06/12 00:00 [accepted]
PHST- 2013/08/08 06:00 [entrez]
PHST- 2013/08/08 06:00 [pubmed]
PHST- 2014/04/30 06:00 [medline]
PHST- 2013/07/26 00:00 [pmc-release]
AID - PONE-D-13-15132 [pii]
AID - 10.1371/journal.pone.0069624 [doi]
PST - epublish
SO  - PLoS One. 2013 Jul 26;8(7):e69624. doi: 10.1371/journal.pone.0069624. Print 2013.