PMID- 23923076
OWN - NLM
STAT- MEDLINE
DCOM- 20140321
LR  - 20211021
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 6
IP  - 8
DP  - 2013
TI  - Butyrate alleviates metabolic impairments and protects pancreatic beta cell function 
      in pregnant mice with obesity.
PG  - 1574-84
AB  - The relative or absolute deficiency of pancreatic beta-cell mass function underlies 
      the pathogenesis of diabetes. It is necessary to alleviate the metabolic stress 
      and reduce the demand for insulin to decrease the effects of mutations affecting 
      beta-cell expansion. Butyrate is a natural nutrient existed in food and can also be 
      produced physiologically through the intestinal fermentation of fiber. Pregnancy 
      and obesity model would be helpful for understanding how beta-cell adapt to insulin 
      resistance and how butyrate alleviate the metabolic impairment and protect 
      pancreatic beta cell function in pregnant mice with obesity. C57BL/6J female mice 
      were divided into three groups and fed with high fat food (HF group, 40% energy 
      from fat), high fat with sodium butyrate food (HSF group, 95% HF with 5% 
      butyrate), or control food (CF group, 14% energy from fat), respectively. The 
      feeding would last for 14 weeks before mating and throughout the gestation 
      period. A subset of dams were sacrificed at gestational day (GD) 14.5 to evaluate 
      the changes of metabolism and beta-cell function, mass, proliferation and apoptosis, 
      inflammatory reaction of islet from different diet. Pancreases were double 
      immuno-labeled to assess the islet morphology, insulin expression, expression of 
      proliferation gene PCNA and anti-apoptosis gene bcl-2. Moreover, we detected the 
      expression of NF-kappaB, phosphorylated NF-kappaB (pNF-kappaB) to evaluate the islet 
      inflammatory response with immunohistochemistry. Mice fed with HSF showed 
      obviously changes including the decreased values of weight gain, glucose, 
      insulin, triglyceride and total cholesterol level of blood compared with high fat 
      diet group, and the reduced circulating maternal pro-inflammation factors at 
      GD14.5. Mice fed with HF displayed beta-cell hyperplasia with a greater beta-cell size 
      and beta-cell area in pancreas. Furthermore, the higher ratio of apoptosis and 
      inflammatory response were found in HF group compared with HSF and CF group, 
      while the proliferation rates of beta-cell increased in HF group, but not in HSF or 
      CF. Butyrate shows an obvious function of anti-obesity, and can alleviate the 
      metabolic stress, maintain the beta-cell function and protect them from inflammatory 
      response in pregnant obese mouse without obvious fetus toxicity.
FAU - Li, Hua-Ping
AU  - Li HP
AD  - Department of Gynecology & Obstetrics, the 6th People's Hospital affiliated to 
      Shanghai Jiaotong University, Shanghai 200233, China. hpli819@sohu.com
FAU - Chen, Xuan
AU  - Chen X
FAU - Li, Ming-Qing
AU  - Li MQ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130715
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - 0 (Butyrates)
SB  - IM
MH  - Animals
MH  - Butyrates/*metabolism/pharmacology
MH  - Diet, High-Fat/adverse effects
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Insulin Resistance/*physiology
MH  - Insulin-Secreting Cells/drug effects/*metabolism/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Obesity/*metabolism
MH  - Pregnancy
MH  - Pregnancy Complications/*metabolism
PMC - PMC3726973
OTO - NOTNLM
OT  - GDM
OT  - Sodium butyrate
OT  - diabetes
OT  - pancreatic islet
OT  - beta-cell
EDAT- 2013/08/08 06:00
MHDA- 2014/03/22 06:00
PMCR- 2013/07/15
CRDT- 2013/08/08 06:00
PHST- 2013/06/18 00:00 [received]
PHST- 2013/07/08 00:00 [accepted]
PHST- 2013/08/08 06:00 [entrez]
PHST- 2013/08/08 06:00 [pubmed]
PHST- 2014/03/22 06:00 [medline]
PHST- 2013/07/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2013 Jul 15;6(8):1574-84. Print 2013.