PMID- 23926537
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130808
LR  - 20211021
IS  - 2228-5482 (Print)
IS  - 2251-676X (Electronic)
IS  - 2228-5482 (Linking)
VI  - 13
IP  - 3
DP  - 2012 Jul
TI  - RHAMM Expression in the Rat Endometrium during the Estrous Cycle and following 
      Implantation.
PG  - 131-7
AB  - BACKGROUND: Receptor for hyaluronic acid mediated motility (RHAMM) has 
      intracellular and extracellular functions. In this study, we focus on the 
      expression of RHAMM in the rat uterus during estrous cycle and implantation 
      period. METHODS: The female adult rats were divided into six groups following 
      estrous cycle determination (n = 36). The utreri of rats were collected according 
      to estrous cycle phases (menstruation group). For the implantation groups, uteri 
      were obtained on D4, D5 and D6 (day of implantation) of pregnancy. The tissue 
      samples were fixed and cut into 5 microm thick sections. RHAMM was investigated using 
      immunohisto-chemical techniques and the intensity of RHAMM was evaluated by using 
      the H-score technique. Comparisons between groups were performed using 
      Kruskal-Wallis test. RESULTS: The RHAMM immunoreactivity of uterine 
      antimesometrial epithelium (343.00+/-12.81), mesometrial subepithelium 
      (285.00+/-27.26) and mesometrial stroma (270.00+/-36.00) were more prominent (p < 
      0.05) in the proestrus than estrus (275.00+/- 25.96; 220.00+/-14.48; 218.00+/-11.19) 
      and diestrus (262.00+/-20.71; 192.50+/- 29.25; 216.00+/-12.97) groups, respectively. 
      The most intense staining was seen in the epithelium on day four (275.50+/-30.06) 
      and six (293.50+/-34.47) of pregnancy (p < 0.05). Strong RHAMM expressions were in 
      both mature and predecidual cells on D5 (256.00+/-18.71), (247.50+/-22.14) and D6 
      (256.00+/-30.72), (265.00+/-14.87), respectively. RHAMM expression was prominent in 
      the nondecidual region on D5 (270.00+/- 13.36). CONCLUSION: Considering the role of 
      RHAMM in cell proliferation, differentiation and angiogenesis, spatiotemporal 
      expression of RHAMM in the uterus during estrous cycle and peri-implantation 
      period is a means through which uterus becomes receptive for developing an 
      embryo.
FAU - Ozbilgin, Kemal
AU  - Ozbilgin K
AD  - Department of Histology and Embryology, School of Medicine, Celal Bayar 
      University, Manisa, Turkey.
FAU - Boz, Banu
AU  - Boz B
FAU - Tugyan, Kazim
AU  - Tugyan K
FAU - Inan, Sevinc
AU  - Inan S
FAU - Vatansever, Seda
AU  - Vatansever S
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - J Reprod Infertil
JT  - Journal of reproduction & infertility
JID - 101535586
PMC - PMC3719349
OTO - NOTNLM
OT  - Estrous cycle
OT  - Immunohistochemistry
OT  - Implantation
OT  - RHAMM
OT  - Uterus
EDAT- 2013/08/09 06:00
MHDA- 2013/08/09 06:01
PMCR- 2012/07/01
CRDT- 2013/08/09 06:00
PHST- 2012/01/15 00:00 [received]
PHST- 2012/05/20 00:00 [accepted]
PHST- 2013/08/09 06:00 [entrez]
PHST- 2013/08/09 06:00 [pubmed]
PHST- 2013/08/09 06:01 [medline]
PHST- 2012/07/01 00:00 [pmc-release]
AID - JRI-13-131 [pii]
PST - ppublish
SO  - J Reprod Infertil. 2012 Jul;13(3):131-7.