PMID- 2392725
OWN - NLM
STAT- MEDLINE
DCOM- 19901004
LR  - 20140912
IS  - 0256-9574 (Print)
VI  - 78
IP  - 5
DP  - 1990 Sep 1
TI  - The sensitivity of tests to detect in vivo platelet activation induced by the 
      removal of arterial endothelium of the baboon (Papio ursinus).
PG  - 271-6
AB  - The relative sensitivities for the various in vivo and in vitro tests for 
      platelet activation are unknown. This was studied in a baboon model where limited 
      and more substantial injury to the vascular endothelium was inflicted. The 
      endothelium of a segment of the right carotid artery was removed with a balloon 
      catheter on day 0 (limited de-endothelialisation), and that of the left carotid 
      artery, abdominal aorta and left femoral artery on day 7 (substantial 
      de-endothelialisation). Eight baboons (Papio ursinus) were used. Baseline tests 
      for platelet activation (platelet volume, platelet density, platelet aggregate 
      ratio, and platelet and plasma levels of platelet factor 4 [PF4] and 
      beta-thromboglobulin [beta-TG]) were performed 7 days before 
      de-endothelialisation and repeated on days 1, 9 and 16. The kinetics of 
      indium-111-labelled platelets were measured after substantial 
      de-endothelialisation. Sham operations were done on 3 animals exactly as in the 
      test, except that the balloon injuries were not inflicted. No influence on the 
      results of the platelet function tests was found. The only test capable of 
      detecting limited injury to the endothelium was the measurement of plasma PF4. 
      The mean platelet life-span (MPLS) shortened, mean platelet density decreased, 
      the circulating platelet aggregate ratio decreased, and plasma levels of PF4 and 
      beta-TG increased (P less than 0.05 in all instances) after the substantial 
      endothelial injury. The mean platelet volume, intraplatelet PF4 and beta-TG, and 
      the in vivo distribution and sites of sequestration of labelled platelets were 
      poor tests for in vivo platelet activation.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Kotze, H F
AU  - Kotze HF
AD  - Department of Haematology, University of the Orange Free State, Bloemfontein.
FAU - Heyns, A D
AU  - Heyns AD
FAU - van Wyk, V
AU  - van Wyk V
FAU - Steyn, J G
AU  - Steyn JG
FAU - Duyvene de Wit, L J
AU  - Duyvene de Wit LJ
FAU - Badenhorst, P N
AU  - Badenhorst PN
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - South Africa
TA  - S Afr Med J
JT  - South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde
JID - 0404520
SB  - IM
MH  - Animals
MH  - Endothelium, Vascular/*physiology
MH  - Papio
MH  - *Platelet Activation
MH  - Platelet Function Tests
MH  - Time Factors
EDAT- 1990/09/01 00:00
MHDA- 1990/09/01 00:01
CRDT- 1990/09/01 00:00
PHST- 1990/09/01 00:00 [pubmed]
PHST- 1990/09/01 00:01 [medline]
PHST- 1990/09/01 00:00 [entrez]
PST - ppublish
SO  - S Afr Med J. 1990 Sep 1;78(5):271-6.