PMID- 23927874
OWN - NLM
STAT- MEDLINE
DCOM- 20140331
LR  - 20130909
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 35
IP  - 4
DP  - 2013 Oct
TI  - Cytokine expression in head-kidney leucocytes of European sea bass (Dicentrarchus 
      labrax L.) after incubation with the probiotic Vagococcus fluvialis L-21.
PG  - 1329-32
LID - S1050-4648(13)00674-8 [pii]
LID - 10.1016/j.fsi.2013.07.036 [doi]
AB  - The European sea bass (Dicentrarchus labrax L.) is one of the most extensively 
      farmed marine fish in the Mediterranean sea. Under the high-density condition, 
      common in aquaculture, the infectious diseases can cause significant economic 
      losses. Probiotics are presented as an alternative to antibiotics for the control 
      of aquaculture diseases. This study used real-time PCR to investigate in vitro 
      the dynamic of expression of immune-related genes in sea bass after incubation 
      with live and inactivated (heat and Uv-light) probiotic Vagoccus fluvialis L-21 
      at different times (T1, T12, T24, T48). The immune associated genes, 
      interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin 10 (IL-10), TumourTumour 
      necrosis factor- (TNF-), ciclo-oxigenase-2 (COX-2), caspase-3 (Casp-3) and Mx 
      were studied in head-kidney (HK) leucocytes of sea bass after incubation with the 
      probiotic strain. Transcript of proinflammatory cytokines (IL-1, TNF-, COX-2) was 
      highly up-regulated after 1 h of incubation with the probiotic strain V. 
      fluvialis L-21. We found statistically significant difference in pick of 
      expression of TNF-, after 1 h of incubation with Uv-light inactivated probiotic 
      strain. The COX-2 expression was highly up-regulated at all times studied, with 
      the exception of 12 and 24 h post incubation for the Uv-light inactivated 
      bacteria. Transcript of IL-10 and Casp-3 showed the higher statistically 
      significant differences of expression after 48 h post incubation with live 
      bacteria. In the contrast, sea bass HK leucocytes expressed Mx at 12 and 48 h 
      without statistically differences among treatments. Our results suggest that V. 
      fluvialis L-21 is able to stimulate in vitro some immune-related genes associated 
      with the early inflammatory response. Future studies in vivo are necessary to 
      clarify this process in sea bass.
CI  - Crown Copyright (c) 2013. Published by Elsevier Ltd. All rights reserved.
FAU - Roman, L
AU  - Roman L
AD  - Instituto Universitario de Sanidad Animal, Universidad de Las Palmas de Gran 
      Canaria, Trasmontana s/n, 35413 Arucas, Spain.
FAU - Real, F
AU  - Real F
FAU - Padilla, D
AU  - Padilla D
FAU - El Aamri, F
AU  - El Aamri F
FAU - Deniz, S
AU  - Deniz S
FAU - Grasso, V
AU  - Grasso V
FAU - Acosta, F
AU  - Acosta F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130806
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Cytokines)
RN  - 0 (Fish Proteins)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Aquaculture
MH  - Bass/*genetics/*immunology/metabolism
MH  - Cytokines/*genetics/immunology
MH  - Enterococcaceae/*chemistry
MH  - Fish Proteins/*genetics/immunology
MH  - Gene Expression Regulation/drug effects
MH  - Head Kidney/cytology
MH  - Immunity, Innate/drug effects
MH  - Leukocytes/metabolism
MH  - Probiotics/*administration & dosage
MH  - Real-Time Polymerase Chain Reaction/veterinary
OTO - NOTNLM
OT  - Cytokine expression
OT  - Leucocytes
OT  - Probiotic
OT  - Sea bass
OT  - Vagococcus fluvialis L-21
EDAT- 2013/08/10 06:00
MHDA- 2014/04/01 06:00
CRDT- 2013/08/10 06:00
PHST- 2013/01/10 00:00 [received]
PHST- 2013/06/18 00:00 [revised]
PHST- 2013/07/21 00:00 [accepted]
PHST- 2013/08/10 06:00 [entrez]
PHST- 2013/08/10 06:00 [pubmed]
PHST- 2014/04/01 06:00 [medline]
AID - S1050-4648(13)00674-8 [pii]
AID - 10.1016/j.fsi.2013.07.036 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2013 Oct;35(4):1329-32. doi: 10.1016/j.fsi.2013.07.036. 
      Epub 2013 Aug 6.