PMID- 23928045
OWN - NLM
STAT- MEDLINE
DCOM- 20140528
LR  - 20131014
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 442
IP  - 2
DP  - 2013 Nov 15
TI  - Penicillin-binding protein 3 of Streptococcus pneumoniae and its application in 
      screening of beta-lactams in milk.
PG  - 158-65
LID - S0003-2697(13)00360-6 [pii]
LID - 10.1016/j.ab.2013.07.042 [doi]
AB  - The soluble form of penicillin-binding protein 3 (sPBP3( *)) from Streptococcus 
      pneumoniae was expressed in Escherichia coli as a six-histidine fusion protein. 
      The protein was purified and used to develop a microplate assay in direct 
      competitive format for the detection of penicillins and cephalosporins in milk. 
      The assay was based on competitive inhibition of the binding of horseradish 
      peroxidase-labeled ampicillin (HRP-Amp) to the sPBP3( *) by free beta-lactam 
      antibiotics in milk. Under optimized conditions, most of the beta-lactam antibiotics 
      (11 penicillins and 16 cephalosporins) could be detected at concentrations 
      corresponding to the maximum residue limits (MRLs) set by the European Union. 
      Analysis of spiked milk samples showed that acceptable recoveries ranged from 
      74.06 to 106.31% in skimmed milk and from 63.97 to 107.26% in whole milk, with 
      coefficients of variation (CVs) less than 16%. With the high sensitivity and 
      wide-range affinities to penicillins and cephalosporins, the developed assay 
      based on sPBP3( *) exhibited the potential to be a screening assay for fast 
      detection of beta-lactam antibiotics in milk.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Zhang, Jing
AU  - Zhang J
AD  - College of Veterinary Medicine, China Agricultural University, Beijing 100094, 
      People's Republic of China; National Reference Laboratories for Veterinary Drug 
      Residue, District Haidian, Beijing 100193, People's Republic of China.
FAU - Wang, Zhanhui
AU  - Wang Z
FAU - Wen, Kai
AU  - Wen K
FAU - Liang, Xiao
AU  - Liang X
FAU - Shen, Jianzhong
AU  - Shen J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130806
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 0 (Bacterial Proteins)
RN  - 0 (Penicillin-Binding Proteins)
RN  - 0 (beta-Lactams)
RN  - 7C782967RD (Ampicillin)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
SB  - IM
MH  - Ampicillin/metabolism
MH  - Animals
MH  - Bacterial Proteins/chemistry/*metabolism
MH  - Binding, Competitive
MH  - Biosensing Techniques/*methods
MH  - Food Analysis/*methods
MH  - Food Contamination/analysis
MH  - Horseradish Peroxidase/metabolism
MH  - Milk/*chemistry
MH  - Penicillin-Binding Proteins/chemistry/*metabolism
MH  - Solubility
MH  - *Streptococcus pneumoniae
MH  - beta-Lactams/*analysis/metabolism
OTO - NOTNLM
OT  - Cephalosporins
OT  - Microplate receptor assay
OT  - Milk
OT  - Penicillin-binding protein 3
OT  - Penicillins
OT  - Residue
EDAT- 2013/08/10 06:00
MHDA- 2014/05/29 06:00
CRDT- 2013/08/10 06:00
PHST- 2013/06/24 00:00 [received]
PHST- 2013/07/29 00:00 [accepted]
PHST- 2013/08/10 06:00 [entrez]
PHST- 2013/08/10 06:00 [pubmed]
PHST- 2014/05/29 06:00 [medline]
AID - S0003-2697(13)00360-6 [pii]
AID - 10.1016/j.ab.2013.07.042 [doi]
PST - ppublish
SO  - Anal Biochem. 2013 Nov 15;442(2):158-65. doi: 10.1016/j.ab.2013.07.042. Epub 2013 
      Aug 6.