PMID- 23928732
OWN - NLM
STAT- MEDLINE
DCOM- 20140603
LR  - 20211203
IS  - 1476-5500 (Electronic)
IS  - 0929-1903 (Linking)
VI  - 20
IP  - 8
DP  - 2013 Aug
TI  - Mst1 overexpression inhibited the growth of human non-small cell lung cancer in 
      vitro and in vivo.
PG  - 453-60
LID - 10.1038/cgt.2013.40 [doi]
AB  - Mammalian STE20-like kinase 1 (Mst1) ubiquitously encodes serine threonine 
      kinase, which is a 59-kDa class II GC kinase that shares 76% identity in 
      amino-acid sequence with MST2, and is the closest mammalian homolog of Drosophila 
      Hippo protein kinase, a major inhibitor of cell proliferation in Drosophila. 
      Recent studies have shown that Mst1 and Mst2 perform tumor-suppressor function in 
      a redundant manner and were originally identified as pro-apoptotic cytoplasmic 
      kinases important for controlling cell growth, proliferation, apoptosis and organ 
      size. We used recombinant eukaryotic expression vector containing human wild-type 
      Mst1 gene to transfect human non-small cell lung cancer (NSCLC) A549 cells in 
      vitro and in vivo. The results showed that Mst1 overexpression inhibited cell 
      proliferation and induced apoptosis of A549 cells, promoted Yes-associated 
      protein (YAP) (Ser127) phosphorylation and downregulated the transcriptional 
      level of Cystein-rich protein connective tissue growth factor (CTGF), 
      amphiregulin (AREG) and Survivin. In human NSCLC-cell-A549-xenograft models, Mst1 
      gene or cisplatin alone suppressed the growth of tumors and increased the 
      cytoplasm-positive expression levels of YAP and Phospho-YAP (Ser127) proteins; 
      however, their combination had the strongest anticancer effects. Overall, Mst1 
      has an important role in inhibiting the growth of NSCLC in vitro and in vivo; its 
      antiproliferative effect is associated with induction of apoptosis through 
      promotion of the cytoplasmic localization and phosphorylation of YAP protein at 
      Ser127 site, indicating that Mst1 may be developed as a promising therapeutic 
      target for NSCLC.
FAU - Xu, C M
AU  - Xu CM
AD  - Department of Biochemistry and Molecular Biology, Basic Medical College of 
      Nanchang University, Nanchang, China.
FAU - Liu, W W
AU  - Liu WW
FAU - Liu, C J
AU  - Liu CJ
FAU - Wen, C
AU  - Wen C
FAU - Lu, H F
AU  - Lu HF
FAU - Wan, F S
AU  - Wan FS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130809
PL  - England
TA  - Cancer Gene Ther
JT  - Cancer gene therapy
JID - 9432230
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (Areg protein, mouse)
RN  - 0 (BIRC5 protein, human)
RN  - 0 (CCN2 protein, human)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Survivin)
RN  - 139568-91-5 (Connective Tissue Growth Factor)
RN  - EC 2.7.1.11 (STK4 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amphiregulin
MH  - Animals
MH  - Apoptosis/physiology
MH  - Carcinoma, Non-Small-Cell Lung/*enzymology/genetics/pathology
MH  - Cell Growth Processes/physiology
MH  - Connective Tissue Growth Factor/biosynthesis/genetics
MH  - EGF Family of Proteins
MH  - Female
MH  - Gene Transfer Techniques
MH  - Glycoproteins/biosynthesis/genetics
MH  - Heterografts
MH  - Humans
MH  - Inhibitor of Apoptosis Proteins/biosynthesis/genetics
MH  - Intercellular Signaling Peptides and Proteins/biosynthesis/genetics
MH  - Intracellular Signaling Peptides and Proteins
MH  - Lung Neoplasms/*enzymology/genetics/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Molecular Sequence Data
MH  - Protein Serine-Threonine Kinases/*biosynthesis/genetics
MH  - Signal Transduction
MH  - Survivin
MH  - Transfection
EDAT- 2013/08/10 06:00
MHDA- 2014/06/04 06:00
CRDT- 2013/08/10 06:00
PHST- 2013/05/15 00:00 [received]
PHST- 2013/05/19 00:00 [accepted]
PHST- 2013/08/10 06:00 [entrez]
PHST- 2013/08/10 06:00 [pubmed]
PHST- 2014/06/04 06:00 [medline]
AID - cgt201340 [pii]
AID - 10.1038/cgt.2013.40 [doi]
PST - ppublish
SO  - Cancer Gene Ther. 2013 Aug;20(8):453-60. doi: 10.1038/cgt.2013.40. Epub 2013 Aug 
      9.