PMID- 23929095
OWN - NLM
STAT- MEDLINE
DCOM- 20140331
LR  - 20211021
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 1048
DP  - 2013
TI  - Analysis of biomarkers of caloric restriction in aging cells.
PG  - 19-29
LID - 10.1007/978-1-62703-556-9_3 [doi]
AB  - Caloric restriction (CR) has been extensively documented for its profound role in 
      effectively extending maximum lifespan in many different species. However, the 
      accurate mechanisms, especially at the cellular level, for CR-induced aging delay 
      are still under intense investigation. An emerging technique, recently explored 
      in our laboratory, provides precisely controllable caloric intake in a cultured 
      cellular system that allows real-time observation and quantitative analysis of 
      the impact of CR on the molecular cellular level during the aging processes. This 
      in vitro method allows investigation of the molecular mechanisms pertaining to 
      how CR influences aging processes leading to life extension in human cellular 
      systems. It will provide important clinical implications for future preventive 
      approaches for aging and aging-related degeneration diseases in humans. Hence, we 
      will discuss the detailed procedures of this novel technique as well as the 
      analysis of relevant aging biomarkers and its broad application in the field.
FAU - Li, Yuanyuan
AU  - Li Y
AD  - Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA.
FAU - Tollefsbol, Trygve O
AU  - Tollefsbol TO
LA  - eng
GR  - P30 CA013148/CA/NCI NIH HHS/United States
GR  - R01 CA129415/CA/NCI NIH HHS/United States
GR  - CA 129415/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Biomarkers)
RN  - EC 3.2.1.23 (beta-Galactosidase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Aging
MH  - Biomarkers/*analysis
MH  - Caloric Restriction/*methods
MH  - Cell Line
MH  - Cell Proliferation
MH  - Cellular Senescence/*physiology
MH  - Fibroblasts/cytology
MH  - Glucose/metabolism
MH  - Humans
MH  - Life Expectancy
MH  - Longevity
MH  - Oxidative Stress
MH  - beta-Galactosidase/*chemistry
PMC - PMC3875466
MID - NIHMS538868
EDAT- 2013/08/10 06:00
MHDA- 2014/04/01 06:00
PMCR- 2013/12/30
CRDT- 2013/08/10 06:00
PHST- 2013/08/10 06:00 [entrez]
PHST- 2013/08/10 06:00 [pubmed]
PHST- 2014/04/01 06:00 [medline]
PHST- 2013/12/30 00:00 [pmc-release]
AID - 10.1007/978-1-62703-556-9_3 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;1048:19-29. doi: 10.1007/978-1-62703-556-9_3.