PMID- 23930008
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130809
LR  - 20211021
IS  - 0973-1296 (Print)
IS  - 0976-4062 (Electronic)
IS  - 0973-1296 (Linking)
VI  - 9
IP  - 35
DP  - 2013 Jul
TI  - Fingerprint analysis of Hibiscus mutabilis L. leaves based on ultra performance 
      liquid chromatography with photodiode array detector combined with similarity 
      analysis and hierarchical clustering analysis methods.
PG  - 238-43
LID - 10.4103/0973-1296.113277 [doi]
AB  - BACKGROUND: A method for chemical fingerprint analysis of Hibiscus mutabilis L. 
      leaves was developed based on ultra performance liquid chromatography with 
      photodiode array detector (UPLC-PAD) combined with similarity analysis (SA) and 
      hierarchical clustering analysis (HCA). MATERIALS AND METHODS: 10 batches of 
      Hibiscus mutabilis L. leaves samples were collected from different regions of 
      China. UPLC-PAD was employed to collect chemical fingerprints of Hibiscus 
      mutabilis L. leaves. RESULTS: The relative standard deviations (RSDs) of the 
      relative retention times (RRT) and relative peak areas (RPA) of 10 characteristic 
      peaks (one of them was identified as rutin) in precision, repeatability and 
      stability test were less than 3%, and the method of fingerprint analysis was 
      validated to be suitable for the Hibiscus mutabilis L. leaves. CONCLUSIONS: The 
      chromatographic fingerprints showed abundant diversity of chemical constituents 
      qualitatively in the 10 batches of Hibiscus mutabilis L. leaves samples from 
      different locations by similarity analysis on basis of calculating the 
      correlation coefficients between each two fingerprints. Moreover, the HCA method 
      clustered the samples into four classes, and the HCA dendrogram showed the close 
      or distant relations among the 10 samples, which was consistent to the SA result 
      to some extent.
FAU - Liang, Xianrui
AU  - Liang X
AD  - Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of 
      Ministry of Education, College of Pharmaceutical Sciences, Zhejiang University of 
      Technology, Hangzhou, Zhejiang, P. R. China.
FAU - Ma, Meiling
AU  - Ma M
FAU - Su, Weike
AU  - Su W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Pharmacogn Mag
JT  - Pharmacognosy magazine
JID - 101300403
PMC - PMC3732427
OTO - NOTNLM
OT  - Fingerprint
OT  - Hibiscus mutabilis L. leaves
OT  - ultra performance liquid chromatography with photodiode array detector
COIS- Conflict of Interest: No.
EDAT- 2013/08/10 06:00
MHDA- 2013/08/10 06:01
PMCR- 2013/07/01
CRDT- 2013/08/10 06:00
PHST- 2012/04/10 00:00 [received]
PHST- 2012/05/26 00:00 [revised]
PHST- 2013/06/11 00:00 [accepted]
PHST- 2013/08/10 06:00 [entrez]
PHST- 2013/08/10 06:00 [pubmed]
PHST- 2013/08/10 06:01 [medline]
PHST- 2013/07/01 00:00 [pmc-release]
AID - PM-9-238 [pii]
AID - 10.4103/0973-1296.113277 [doi]
PST - ppublish
SO  - Pharmacogn Mag. 2013 Jul;9(35):238-43. doi: 10.4103/0973-1296.113277.