PMID- 23932363
OWN - NLM
STAT- MEDLINE
DCOM- 20140417
LR  - 20211203
IS  - 1872-8332 (Electronic)
IS  - 0169-5002 (Linking)
VI  - 82
IP  - 1
DP  - 2013 Oct
TI  - Diagnostic method for the detection of KIF5B-RET transformation in lung 
      adenocarcinoma.
PG  - 44-50
LID - S0169-5002(13)00318-8 [pii]
LID - 10.1016/j.lungcan.2013.07.009 [doi]
AB  - KIF5B-RET fusions have recently been reported to occur in pulmonary 
      adenocarcinomas, thereby being proposed as a novel genetic alteration in 
      adenocarcinoma of the lung. However, clinically useful methods to detect 
      RET-rearrangement in pulmonary adenocarcinoma have not been well established. 53 
      cases of lung adenocarcinomas harbored "triple (EGFR, KRAS and ALK)-negative" 
      were tested for KIF5B-RET fusions using whole-transcriptome sequencing, 
      fluorescence in situ hybridization (FISH), immunohistochemistry (IHC), and 
      long-range PCR. Dual color break-apart probes and KIF5B-RET fusion probes were 
      used for FISH. Three different commercial antibodies against C-terminal RET 
      protein were tested for IHC. Primers designed for 3 different variants of 
      KIF5B-RET fusions were used for long-range PCR. Three patients (5.6%) showed RET 
      rearrangement in whole-transcriptome sequencing, which were used as a gold 
      standard. All those three patients were also positive in FISH for both KIF5B-RET 
      fusion and RET break-apart probes. None of remaining patients showed positive 
      result, resulting in 100% concordance rate of FISH and transcriptome sequencing 
      methods. However, fused RET proteins were not detected by IHC in none of true 
      positive patients. Moreover, 6 patients without RET fusions showed gain of gene 
      copy number of both KIF5B and RET. All those three true positive cases were 
      detected by long-range PCR methods and none with true negative cases were 
      positive. Both FISH and PCR may be useful methods to detect novel KIF5B-RET 
      rearrangements in pulmonary adenocarcinomas rather than IHC. However, as there 
      may be additional variant of fusion mutation, FISH may be better than PCR method 
      in terms of sensitivity.
CI  - Copyright (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Go, Heounjeong
AU  - Go H
AD  - Department of Pathology, Seoul National University College of Medicine, Seoul, 
      Republic of Korea.
FAU - Jung, Yoo Jin
AU  - Jung YJ
FAU - Kang, Hyun Woong
AU  - Kang HW
FAU - Park, In-Kyu
AU  - Park IK
FAU - Kang, Chang-Hyun
AU  - Kang CH
FAU - Lee, Jung Wan
AU  - Lee JW
FAU - Ju, Young Seok
AU  - Ju YS
FAU - Seo, Jeong-Sun
AU  - Seo JS
FAU - Chung, Doo Hyun
AU  - Chung DH
FAU - Kim, Young Tae
AU  - Kim YT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130809
PL  - Ireland
TA  - Lung Cancer
JT  - Lung cancer (Amsterdam, Netherlands)
JID - 8800805
RN  - 0 (KIF5B protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-ret)
RN  - EC 2.7.10.1 (RET protein, human)
RN  - EC 3.6.4.4 (Kinesins)
SB  - IM
MH  - Adenocarcinoma/*diagnosis/genetics
MH  - Adenocarcinoma of Lung
MH  - Aged
MH  - DNA Mutational Analysis
MH  - Female
MH  - Humans
MH  - Kinesins/*genetics
MH  - Lung Neoplasms/*diagnosis/genetics
MH  - Male
MH  - Middle Aged
MH  - Molecular Diagnostic Techniques
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Polymerase Chain Reaction
MH  - Proto-Oncogene Proteins c-ret/*genetics
MH  - Sequence Analysis, DNA
MH  - Transcriptome
OTO - NOTNLM
OT  - Biomarkers
OT  - Fluorescence in situ hybridization (FISH)
OT  - Immunohistochemistry (IHC)
OT  - Lung cancer
OT  - Polymerase chain reaction (PCR)
EDAT- 2013/08/13 06:00
MHDA- 2014/04/18 06:00
CRDT- 2013/08/13 06:00
PHST- 2013/03/30 00:00 [received]
PHST- 2013/06/29 00:00 [revised]
PHST- 2013/07/11 00:00 [accepted]
PHST- 2013/08/13 06:00 [entrez]
PHST- 2013/08/13 06:00 [pubmed]
PHST- 2014/04/18 06:00 [medline]
AID - S0169-5002(13)00318-8 [pii]
AID - 10.1016/j.lungcan.2013.07.009 [doi]
PST - ppublish
SO  - Lung Cancer. 2013 Oct;82(1):44-50. doi: 10.1016/j.lungcan.2013.07.009. Epub 2013 
      Aug 9.