PMID- 23932907
OWN - NLM
STAT- MEDLINE
DCOM- 20140606
LR  - 20131028
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Linking)
VI  - 193
DP  - 2013 Nov 1
TI  - Identification, characterization, and expression profiles of two subtypes of 
      kisspeptin receptors in a scombroid fish (chub mackerel).
PG  - 130-40
LID - S0016-6480(13)00320-1 [pii]
LID - 10.1016/j.ygcen.2013.07.016 [doi]
AB  - The kisspeptin receptor (Kiss1R) is a cognate receptor for kisspeptin (Kiss), and 
      this Kiss-Kiss1R system has been shown to regulate seasonal reproduction in 
      vertebrates. Our previous study found the chub mackerel (Scomber japonicus) brain 
      expresses both kiss1 and kiss2 and exhibits sexually dimorphic changes during the 
      seasonal reproductive cycle. The present study cloned two subtypes of kissr from 
      the chub mackerel brain, and their signal transduction pathways to Kiss1 and 
      Kiss2 were characterized in a mammalian cell line. Results of identification 
      showed that kissr1 and kissr2 mRNAs encode 369 and 378 deduced amino acids, 
      respectively, and share 52% similarity in amino acid sequences. In vitro 
      functional analysis revealed that chub mackerel Kiss receptor signals are also 
      preferentially transduced via the protein kinase C (PKC) rather than protein 
      kinase A (PKA) pathway. Synthetic chub mackerel Kiss1-15 and Kiss2-12 peptides 
      showed the highest potency for the activation of KissR1 and KissR2, respectively, 
      stronger than their corresponding Kiss-10 peptides. Tissue distribution analyses 
      indicated that both genes are highly expressed in the brain and that only kissr2 
      mRNA is expressed in the pituitary of both sexes. Unexpectedly, both kissr1 and 
      kissr2 mRNAs were detected only in the testes. Seasonal expression changes showed 
      higher expression levels of both kissr1 and kissr2 mRNAs in the brain of females 
      during the early vitellogenic period; however, no significant differences were 
      found in the brain of males. Pituitary kissr2 mRNA levels showed no significant 
      variations. In the testes, the kissr1 mRNA expression level increased 
      dramatically at spermiation compared with the immature and post-spawning periods. 
      However, kissr2 mRNA levels in the testes did not vary significantly at different 
      testicular stages. These results suggest that both kissr1 and kissr2 likely 
      participate in the seasonal ovarian development of females, and thus in males, we 
      propose a paracrine or autocrine role for kissr1 in testicular development.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Ohga, Hirofumi
AU  - Ohga H
AD  - Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 
      812-8581, Japan.
FAU - Fujinaga, Yoichiro
AU  - Fujinaga Y
FAU - Selvaraj, Sethu
AU  - Selvaraj S
FAU - Kitano, Hajime
AU  - Kitano H
FAU - Nyuji, Mitsuo
AU  - Nyuji M
FAU - Yamaguchi, Akihiko
AU  - Yamaguchi A
FAU - Matsuyama, Michiya
AU  - Matsuyama M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130809
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (Fish Proteins)
RN  - 0 (Kisspeptins)
RN  - 0 (Receptors, G-Protein-Coupled)
SB  - IM
MH  - Animals
MH  - Brain/metabolism/physiology
MH  - Female
MH  - Fish Proteins/genetics/*metabolism
MH  - Kisspeptins/metabolism
MH  - Male
MH  - Perciformes/*metabolism/physiology
MH  - Receptors, G-Protein-Coupled/genetics/*metabolism
MH  - Reproduction/physiology
MH  - Testis/metabolism/physiology
OTO - NOTNLM
OT  - GPR54
OT  - Kiss1r
OT  - Perciformes
EDAT- 2013/08/13 06:00
MHDA- 2014/06/07 06:00
CRDT- 2013/08/13 06:00
PHST- 2012/11/12 00:00 [received]
PHST- 2013/07/12 00:00 [revised]
PHST- 2013/07/19 00:00 [accepted]
PHST- 2013/08/13 06:00 [entrez]
PHST- 2013/08/13 06:00 [pubmed]
PHST- 2014/06/07 06:00 [medline]
AID - S0016-6480(13)00320-1 [pii]
AID - 10.1016/j.ygcen.2013.07.016 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2013 Nov 1;193:130-40. doi: 10.1016/j.ygcen.2013.07.016. 
      Epub 2013 Aug 9.