PMID- 23933144
OWN - NLM
STAT- MEDLINE
DCOM- 20141218
LR  - 20131101
IS  - 1872-7549 (Electronic)
IS  - 0166-4328 (Linking)
VI  - 256
DP  - 2013 Nov 1
TI  - Melatonin ameliorates cognitive impairment induced by sleep deprivation in rats: 
      role of oxidative stress, BDNF and CaMKII.
PG  - 72-81
LID - S0166-4328(13)00463-4 [pii]
LID - 10.1016/j.bbr.2013.07.051 [doi]
AB  - Sleep deprivation (SD) has been shown to induce oxidative stress which causes 
      cognitive impairment. Melatonin, an endogenous potent antioxidant, protects 
      neurons from oxidative stress in many disease models. The present study 
      investigated the effect of melatonin against SD-induced cognitive impairment and 
      attempted to define the possible mechanisms involved. SD was induced in rats 
      using modified multiple platform model. Melatonin (15 mg/kg) was administered to 
      the rats via intraperitoneal injection. The open field test and Morris water maze 
      were used to evaluate cognitive ability. The cerebral cortex (CC) and hippocampus 
      were dissected and homogenized. Nitric oxide (NO) and malondialdehyde (MDA) 
      levels and the superoxide dismutase (SOD) enzyme activity of hippocampal and 
      cortical tissues (10% wet weight per volume) were performed to determine the 
      level of oxidative stress. The expression of brain-derived neurotrophic factor 
      (BDNF) and calcium-calmodulin dependent kinase II (CaMKII) proteins in CC and 
      hippocampus was assayed by means of immunohistochemistry. The results revealed 
      that SD impairs cognitive ability, while melatonin treatment prevented these 
      changes. In addition, melatonin reversed SD-induced changes in NO, MDA and SOD in 
      both of the CC and hippocampus. The results of immunoreactivity showed that SD 
      decreased gray values of BDNF and CaMKII in CC and hippocamal CA1, CA3 and 
      dentate gyrus regions, whereas melatonin improved the gray values. In conclusion, 
      our results suggest that melatonin prevents cognitive impairment induced by SD. 
      The possible mechanism may be attributed to its ability to reduce oxidative 
      stress and increase the levels of CaMKII and BDNF in CC and hippocampus.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Experiment Teaching Center of Basic Medicine, The Fourth Military Medical 
      University, Xi'an, 710033 Shan Xi Province, China; Department of General Surgery, 
      406 Hospital, Da Lian, 116041 Liao Ning Province, China; State Key Laboratory of 
      Cancer Biology, Xijing Hospital of Digestive Diseases, The Fourth Military 
      Medical University, Xi'an, 710033 Shan Xi Province, China.
FAU - Zhang, Hu-Qin
AU  - Zhang HQ
FAU - Liang, Xiang-Yan
AU  - Liang XY
FAU - Zhang, Hai-Feng
AU  - Zhang HF
FAU - Zhang, Ting
AU  - Zhang T
FAU - Liu, Fang-E
AU  - Liu FE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130806
PL  - Netherlands
TA  - Behav Brain Res
JT  - Behavioural brain research
JID - 8004872
RN  - 0 (Antioxidants)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nootropic Agents)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - JL5DK93RCL (Melatonin)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Brain/*drug effects/metabolism
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism
MH  - Cerebral Cortex/drug effects/metabolism
MH  - Cognition Disorders/*drug therapy/*etiology/metabolism
MH  - Hippocampus/drug effects/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Maze Learning/drug effects/physiology
MH  - Melatonin/*pharmacology
MH  - Nitric Oxide/metabolism
MH  - Nootropic Agents/*pharmacology
MH  - Oxidative Stress/drug effects/physiology
MH  - Rats, Sprague-Dawley
MH  - Sleep Deprivation/*complications
MH  - Superoxide Dismutase/metabolism
OTO - NOTNLM
OT  - BDNF
OT  - CC
OT  - CG
OT  - CaMKII
OT  - Calcium-calmodulin dependent kinase II
OT  - Cognitive impairment
OT  - DG
OT  - DTC
OT  - EL
OT  - FMT
OT  - ITT
OT  - LTP
OT  - MDA
OT  - MSD(1)
OT  - MSD(2)
OT  - Melatonin
OT  - NCP
OT  - NO
OT  - OF
OT  - Oxidative stress
OT  - P-CaMKII
OT  - PBS
OT  - REM
OT  - ROS
OT  - SD
OT  - SD(1)
OT  - SD(2)
OT  - SOD
OT  - Sleep deprivation
OT  - TMD
OT  - WPG(1)
OT  - WPG(2)
OT  - brain-derived neurotrophic factor
OT  - cerebral cortex
OT  - control group
OT  - dentate gyrus
OT  - distance to the center
OT  - escape latency
OT  - faster movement time
OT  - inner toriod time
OT  - long-term potentiation
OT  - malondialdehyde
OT  - melatonin with sleep deprivation 2
OT  - melatonin with sleep deprivation group 1
OT  - nitric oxide
OT  - number of crossings over the former platform location
OT  - open field
OT  - phosphate buffer saline
OT  - phosphorylated Calcium-calmodulin dependent kinase II
OT  - rapid eye movent
OT  - reactive oxygen species
OT  - sleep deprivation
OT  - sleep deprivation 2
OT  - sleep deprivation group 1
OT  - superoxide dismutase
OT  - total movement distance
OT  - wide platform group 1
OT  - wide platform group 2
EDAT- 2013/08/13 06:00
MHDA- 2014/12/19 06:00
CRDT- 2013/08/13 06:00
PHST- 2013/06/22 00:00 [received]
PHST- 2013/07/23 00:00 [revised]
PHST- 2013/07/27 00:00 [accepted]
PHST- 2013/08/13 06:00 [entrez]
PHST- 2013/08/13 06:00 [pubmed]
PHST- 2014/12/19 06:00 [medline]
AID - S0166-4328(13)00463-4 [pii]
AID - 10.1016/j.bbr.2013.07.051 [doi]
PST - ppublish
SO  - Behav Brain Res. 2013 Nov 1;256:72-81. doi: 10.1016/j.bbr.2013.07.051. Epub 2013 
      Aug 6.