PMID- 23936213
OWN - NLM
STAT- MEDLINE
DCOM- 20140401
LR  - 20220331
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 7
DP  - 2013
TI  - Alterations and abnormal mitosis of wheat chromosomes induced by wheat-rye 
      monosomic addition lines.
PG  - e70483
LID - 10.1371/journal.pone.0070483 [doi]
LID - e70483
AB  - BACKGROUND: Wheat-rye addition lines are an old topic. However, the alterations 
      and abnormal mitotic behaviours of wheat chromosomes caused by wheat-rye 
      monosomic addition lines are seldom reported. METHODOLOGY/PRINCIPAL FINDINGS: 
      Octoploid triticale was derived from common wheat T. aestivum L. 'Mianyang11'xrye 
      S. cereale L. 'Kustro' and some progeny were obtained by the controlled 
      backcrossing of triticale with 'Mianyang11' followed by self-fertilization. 
      Genomic in situ hybridization (GISH) using rye genomic DNA and fluorescence in 
      situ hybridization (FISH) using repetitive sequences pAs1 and pSc119.2 as probes 
      were used to analyze the mitotic chromosomes of these progeny. Strong pSc119.2 
      FISH signals could be observed at the telomeric regions of 3DS arms in 
      'Mianyang11'. However, the pSc119.2 FISH signals were disappeared from the selfed 
      progeny of 4R monosomic addition line and the changed 3D chromosomes could be 
      transmitted to next generation stably. In one of the selfed progeny of 7R 
      monosomic addition line, one 2D chromosome was broken and three 4A chromosomes 
      were observed. In the selfed progeny of 6R monosomic addition line, structural 
      variation and abnormal mitotic behaviour of 3D chromosome were detected. 
      Additionally, 1A and 4B chromosomes were eliminated from some of the progeny of 
      6R monosomic addition line. CONCLUSIONS/SIGNIFICANCE: These results indicated 
      that single rye chromosome added to wheat might cause alterations and abnormal 
      mitotic behaviours of wheat chromosomes and it is possible that the stress caused 
      by single alien chromosome might be one of the factors that induced karyotype 
      alteration of wheat.
FAU - Fu, Shulan
AU  - Fu S
AD  - State Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural 
      University, Wenjiang, Chengdu, Sichuan, PR China. fushulan@sicau.edu.cn
FAU - Yang, Manyu
AU  - Yang M
FAU - Fei, Yunyan
AU  - Fei Y
FAU - Tan, Feiquan
AU  - Tan F
FAU - Ren, Zhenglong
AU  - Ren Z
FAU - Yan, Benju
AU  - Yan B
FAU - Zhang, Huaiyu
AU  - Zhang H
FAU - Tang, Zongxiang
AU  - Tang Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130730
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
EIN - PLoS One. 2014;9(8):e106288
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Plant
MH  - Genetic Variation
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - *Mitosis
MH  - Polyploidy
MH  - Secale/*genetics
MH  - Triticum/*genetics
PMC - PMC3728071
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/08/13 06:00
MHDA- 2014/04/02 06:00
PMCR- 2013/07/30
CRDT- 2013/08/13 06:00
PHST- 2013/04/16 00:00 [received]
PHST- 2013/06/19 00:00 [accepted]
PHST- 2013/08/13 06:00 [entrez]
PHST- 2013/08/13 06:00 [pubmed]
PHST- 2014/04/02 06:00 [medline]
PHST- 2013/07/30 00:00 [pmc-release]
AID - PONE-D-13-15336 [pii]
AID - 10.1371/journal.pone.0070483 [doi]
PST - epublish
SO  - PLoS One. 2013 Jul 30;8(7):e70483. doi: 10.1371/journal.pone.0070483. Print 2013.