PMID- 23936250
OWN - NLM
STAT- MEDLINE
DCOM- 20140305
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 7
DP  - 2013
TI  - Dual-color fluorescence in situ hybridization reveals an association of 
      chromosome 8q22 but not 8p21 imbalance with high grade invasive breast carcinoma.
PG  - e70790
LID - 10.1371/journal.pone.0070790 [doi]
LID - e70790
AB  - We previously reported molecular karyotype analysis of invasive breast tumour 
      core needle biopsies by comparative genomic hybridization (CGH) and fluorescence 
      in situ hybridization (FISH) (Walker et al, Genes Chromosomes Cancer, 2008 
      May;47(5):405-17). That study identified frequently recurring gains and losses 
      involving chromosome bands 8q22 and 8p21, respectively. Moreover, these data 
      highlighted an association between 8q22 gain and typically aggressive grade 3 
      tumors. Here we validate and extend our previous investigations through FISH 
      analysis of tumor touch imprints prepared from excised breast tumor specimens. 
      Compared to post-surgical tumor excisions, core needle biopsies are known to be 
      histologically less precise when predicting tumor grade. Therefore investigating 
      these chromosomal aberrations in tumor samples that offer more reliable 
      pathological assessment is likely to give a better overall indication of 
      association. A series of 60 breast tumors were screened for genomic copy number 
      changes at 8q22 and 8p21 by dual-color FISH. Results confirm previous findings 
      that 8p loss (39%) and 8q gain (74%) occur frequently in invasive breast cancer. 
      Both absolute quantification of 8q22 gain across the sample cohort, and a 
      separate relative assessment by 8q22:8p21 copy number ratio, showed that the 
      incidence of 8q22 gain significantly increased with grade (p = 0.004, absolute 
      and p = 0.02, relative). In contrast, no association was found between 8p21 loss 
      and tumor grade. These findings support the notion that 8q22 is a region of 
      interest for invasive breast cancer pathogenesis, potentially harboring one or 
      more genes that, when amplified, precipitate the molecular events that define 
      high tumor grade.
FAU - Walker, Logan C
AU  - Walker LC
AD  - Cancer Genetics Research Group, Department of Pathology, University of Otago, 
      Christchurch, New Zealand. logan.walker@otago.ac.nz
FAU - McDonald, Margaret
AU  - McDonald M
FAU - Wells, J Elisabeth
AU  - Wells JE
FAU - Harris, Gavin C
AU  - Harris GC
FAU - Robinson, Bridget A
AU  - Robinson BA
FAU - Morris, Christine M
AU  - Morris CM
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130725
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Aged
MH  - Breast Neoplasms/*diagnosis/genetics/mortality/surgery
MH  - Carcinoma, Ductal, Breast/*diagnosis/genetics/mortality/surgery
MH  - Chromosome Banding
MH  - *Chromosomes, Human, Pair 8
MH  - DNA Copy Number Variations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Mastectomy
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - Survival Analysis
MH  - *Translocation, Genetic
PMC - PMC3723675
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/08/13 06:00
MHDA- 2014/03/07 06:00
PMCR- 2013/07/25
CRDT- 2013/08/13 06:00
PHST- 2013/03/05 00:00 [received]
PHST- 2013/06/24 00:00 [accepted]
PHST- 2013/08/13 06:00 [entrez]
PHST- 2013/08/13 06:00 [pubmed]
PHST- 2014/03/07 06:00 [medline]
PHST- 2013/07/25 00:00 [pmc-release]
AID - PONE-D-13-09253 [pii]
AID - 10.1371/journal.pone.0070790 [doi]
PST - epublish
SO  - PLoS One. 2013 Jul 25;8(7):e70790. doi: 10.1371/journal.pone.0070790. Print 2013.