PMID- 23940516
OWN - NLM
STAT- MEDLINE
DCOM- 20140305
LR  - 20220223
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 8
DP  - 2013
TI  - Radiation therapy-induced tumor invasiveness is associated with SDF-1-regulated 
      macrophage mobilization and vasculogenesis.
PG  - e69182
LID - 10.1371/journal.pone.0069182 [doi]
LID - e69182
AB  - Radiation therapy (RT) remains the front-line treatment for high-grade gliomas; 
      however, tumor recurrence remains the main obstacle for the clinical success of 
      RT. Using a murine astrocytoma tumor cell line, ALTS1C1, the present study 
      demonstrates that whole brain irradiation prolonged the survival of tumor-bearing 
      mice, although the mice eventually died associated with increased tumor 
      infiltration. Immunohistochemical (IHC) analysis indicated that RT decreased the 
      microvascular density (MVD) of the primary tumor core, but increased the MVD of 
      the tumor invasion front. RT also increased the number of tumor-associated 
      macrophages (TAMs) and the expression of stromal cell-derived factor-1 (SDF-1) 
      and hypoxia-inducible factor-1 (HIF-1) at the tumor invasion front. SDF-1 
      expression suppressed by siRNA (SDFkd tumors) showed a decrease in RT-enhanced 
      tumor invasiveness, leading to prolonged survival of mice bearing these tumors. 
      The invasion front in SDFkd tumors showed a lower MVD and TAM density than that 
      in the islands of the control or irradiated ALTS1C1 tumors. Our results indicate 
      that tumor-secreted SDF-1 is one key factor in RT-induced tumor invasiveness, and 
      that it exerts its effect likely through macrophage mobilization and tumor 
      revascularization.
FAU - Wang, Shu-Chi
AU  - Wang SC
AD  - Department of Biomedical Engineering and Environmental Sciences, National 
      Tsing-Hua University, Hsinchu, Taiwan.
FAU - Yu, Ching-Fang
AU  - Yu CF
FAU - Hong, Ji-Hong
AU  - Hong JH
FAU - Tsai, Chien-Sheng
AU  - Tsai CS
FAU - Chiang, Chi-Shiun
AU  - Chiang CS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130805
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Hypoxia-Inducible Factor 1)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Chemokine CXCL12/*metabolism
MH  - Glioma/*radiotherapy
MH  - Hypoxia-Inducible Factor 1/metabolism
MH  - Immunohistochemistry
MH  - Macrophages/*cytology/metabolism/radiation effects
MH  - Mice
PMC - PMC3734136
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/08/14 06:00
MHDA- 2014/03/07 06:00
PMCR- 2013/08/05
CRDT- 2013/08/14 06:00
PHST- 2013/03/12 00:00 [received]
PHST- 2013/06/05 00:00 [accepted]
PHST- 2013/08/14 06:00 [entrez]
PHST- 2013/08/14 06:00 [pubmed]
PHST- 2014/03/07 06:00 [medline]
PHST- 2013/08/05 00:00 [pmc-release]
AID - PONE-D-13-10962 [pii]
AID - 10.1371/journal.pone.0069182 [doi]
PST - epublish
SO  - PLoS One. 2013 Aug 5;8(8):e69182. doi: 10.1371/journal.pone.0069182. Print 2013.