PMID- 23942118
OWN - NLM
STAT- MEDLINE
DCOM- 20131112
LR  - 20131121
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 439
IP  - 1
DP  - 2013 Sep 13
TI  - Maintenance of cellular ATP level by caloric restriction correlates chronological 
      survival of budding yeast.
PG  - 126-31
LID - S0006-291X(13)01330-2 [pii]
LID - 10.1016/j.bbrc.2013.08.014 [doi]
AB  - The free radical theory of aging emphasizes cumulative oxidative damage in the 
      genome and intracellular proteins due to reactive oxygen species (ROS), which is 
      a major cause for aging. Caloric restriction (CR) has been known as a 
      representative treatment that prevents aging; however, its mechanism of action 
      remains elusive. Here, we show that CR extends the chronological lifespan (CLS) 
      of budding yeast by maintaining cellular energy levels. CR reduced the generation 
      of total ROS and mitochondrial superoxide; however, CR did not reduce the 
      oxidative damage in proteins and DNA. Subsequently, calorie-restricted yeast had 
      higher mitochondrial membrane potential (MMP), and it sustained consistent ATP 
      levels during the process of chronological aging. Our results suggest that CR 
      extends the survival of the chronologically aged cells by improving the 
      efficiency of energy metabolism for the maintenance of the ATP level rather than 
      reducing the global oxidative damage of proteins and DNA.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Choi, Joon-Seok
AU  - Choi JS
AD  - Division of Biotechnology, College of Life Sciences & Biotechnology, Korea 
      University, Seoul 136-701, Republic of Korea.
FAU - Lee, Cheol-Koo
AU  - Lee CK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130811
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Culture Media)
RN  - 11062-77-4 (Superoxides)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adenosine Triphosphate/*chemistry
MH  - Culture Media
MH  - DNA/*metabolism
MH  - Electron Transport
MH  - Energy Metabolism
MH  - Flow Cytometry
MH  - Membrane Potential, Mitochondrial
MH  - Mitochondria/*metabolism
MH  - Oxidative Stress
MH  - Saccharomyces cerevisiae/genetics/*metabolism
MH  - Superoxides/metabolism
MH  - Time Factors
OTO - NOTNLM
OT  - 2',7'-dichlorodihydrofluorescein diacetate
OT  - 3,3'-dihexyloxacarbocyanine iodide
OT  - 8-OHdG
OT  - 8-hydroxydeoxyguanosine
OT  - ATP
OT  - Aging
OT  - Budding yeast
OT  - CLS
OT  - CR
OT  - Caloric restriction
OT  - Chronological lifespan
OT  - DNPH
OT  - DiOC(6)
OT  - ETC
OT  - H2DCFDA
OT  - RLS
OT  - ROS
OT  - caloric restriction
OT  - chronological lifespan
OT  - dinitrophenylhydrazine
OT  - electron transport chain
OT  - reactive oxygen species
OT  - replicative lifespan
EDAT- 2013/08/15 06:00
MHDA- 2013/11/13 06:00
CRDT- 2013/08/15 06:00
PHST- 2013/07/31 00:00 [received]
PHST- 2013/08/05 00:00 [accepted]
PHST- 2013/08/15 06:00 [entrez]
PHST- 2013/08/15 06:00 [pubmed]
PHST- 2013/11/13 06:00 [medline]
AID - S0006-291X(13)01330-2 [pii]
AID - 10.1016/j.bbrc.2013.08.014 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2013 Sep 13;439(1):126-31. doi: 
      10.1016/j.bbrc.2013.08.014. Epub 2013 Aug 11.