PMID- 23943448
OWN - NLM
STAT- MEDLINE
DCOM- 20140317
LR  - 20130814
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1032
DP  - 2013
TI  - Purifying and measuring immunoglobulin E (IgE) and anti-IgE.
PG  - 121-31
LID - 10.1007/978-1-62703-496-8_9 [doi]
AB  - Immunoglobulins (Igs) are a critical component of the adaptive immune system of 
      both man and mouse. The ability to detect and characterize Igs is an invaluable 
      technique for immunology in either a research or a clinical setting. The advent 
      of enzyme-linked immunosorbent assays (ELISAs) and monoclonal antibody technology 
      has proven instrumental for advancing the science of Ig biology. IgE is of 
      interest as it is the primary Ig responsible for allergic reactions ranging from 
      allergic rhinitis to anaphylaxis. Here, we describe the history behind the IgE 
      discovery and the protocol for purifying IgE and anti-IgE in the mouse. This is 
      followed by our ELISA protocol for mouse IgE detection.
FAU - Sturgill, Jamie L
AU  - Sturgill JL
AD  - Department of Microbiology and Immunology, Virginia Commonwealth University 
      School of Medicine, Richmond, VA, USA.
FAU - Conrad, Daniel H
AU  - Conrad DH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (anti-IgE antibodies)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/blood/*isolation & purification
MH  - Antibodies, Monoclonal/immunology
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Humans
MH  - Immunoglobulin E/blood/*isolation & purification
MH  - Mice
EDAT- 2013/08/15 06:00
MHDA- 2014/03/19 06:00
CRDT- 2013/08/15 06:00
PHST- 2013/08/15 06:00 [entrez]
PHST- 2013/08/15 06:00 [pubmed]
PHST- 2014/03/19 06:00 [medline]
AID - 10.1007/978-1-62703-496-8_9 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;1032:121-31. doi: 10.1007/978-1-62703-496-8_9.