PMID- 23944371
OWN - NLM
STAT- MEDLINE
DCOM- 20150423
LR  - 20161020
IS  - 1939-165X (Electronic)
IS  - 0275-6382 (Linking)
VI  - 42
IP  - 3
DP  - 2013 Sep
TI  - Serum paraoxonase 1 activity in dogs: preanalytical and analytical factors and 
      correlation with C-reactive protein and alpha-2-globulin.
PG  - 329-41
LID - 10.1111/vcp.12073 [doi]
AB  - BACKGROUND: Serum activity of paraoxonase (PON1) decreases during inflammation in 
      many species. Little information is available on paraoxon-based tests and the 
      possible role of PON1 in dogs. OBJECTIVES: The objectives of the study were to 
      validate an automated paraoxon-based assay to measure PON1 activity in canine 
      serum, to determine its stability under different storage conditions, to 
      determine a reference interval (RI) in healthy dogs, and to assess whether PON1 
      is of comparable diagnostic value as C-reactive protein (CRP) and alpha2-globulins. 
      METHODS: Intra-assay and inter-assay imprecision, linearity under dilution (LUD), 
      interference, and storage artifacts were evaluated. A PON1 RI was determined for 
      healthy dogs, and PON1 activity, sensitivity, and specificity were compared with 
      CRP and alpha2-globulins. RESULTS: Intra- and inter-assay CVs were below 1.6% and 
      7.8%, respectively. The LUD test fitted the linear model. PON1 activity 
      measurements were increased after addition of hemolysates and lipids, and after 
      storage for 12 hours at room temperature, 72 hours at 4 degrees C, and 6 months at -20 degrees C. 
      PON1 activity and CRP or alpha2-globulins did not correlate well. PON1 activity 
      decreased significantly only in dogs with very high CRP concentrations. In 
      contrast to CRP and alpha2-globulins, PON1 activity was not significantly different 
      between dogs with and without inflammation. CONCLUSIONS: The automated 
      paraoxon-based method to assess serum canine PON1 activity was accurate and 
      precise, but it was influenced by hemolysis, lipemia, and standard storage 
      conditions. In this study, contrarily to CRP and alpha2-globulins, PON1 activity did 
      not provide diagnostic value as a negative acute phase protein in dogs.
CI  - (c) 2013 American Society for Veterinary Clinical Pathology.
FAU - Rossi, Gabriele
AU  - Rossi G
AD  - Department of Veterinary Sciences and Public Health, University of Milan, Milan, 
      Italy.
FAU - Giordano, Alessia
AU  - Giordano A
FAU - Pezzia, Francesca
AU  - Pezzia F
FAU - Kjelgaard-Hansen, Mads
AU  - Kjelgaard-Hansen M
FAU - Paltrinieri, Saverio
AU  - Paltrinieri S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130814
PL  - United States
TA  - Vet Clin Pathol
JT  - Veterinary clinical pathology
JID - 9880575
RN  - 0 (Alpha-Globulins)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - EC 3.1.8.1 (Aryldialkylphosphatase)
SB  - IM
MH  - Alpha-Globulins/analysis/metabolism
MH  - Animals
MH  - Aryldialkylphosphatase/blood/*metabolism
MH  - Automation, Laboratory
MH  - C-Reactive Protein/analysis/metabolism
MH  - Dogs
MH  - Female
MH  - Male
MH  - Reference Values
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - Acute phase protein
OT  - inflammation
OT  - serum protein electrophoresis
OT  - storage
OT  - validation
EDAT- 2013/08/16 06:00
MHDA- 2015/04/24 06:00
CRDT- 2013/08/16 06:00
PHST- 2013/08/16 06:00 [entrez]
PHST- 2013/08/16 06:00 [pubmed]
PHST- 2015/04/24 06:00 [medline]
AID - 10.1111/vcp.12073 [doi]
PST - ppublish
SO  - Vet Clin Pathol. 2013 Sep;42(3):329-41. doi: 10.1111/vcp.12073. Epub 2013 Aug 14.