PMID- 23946637
OWN - NLM
STAT- MEDLINE
DCOM- 20130924
LR  - 20211021
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 19
DP  - 2013
TI  - Increase in peripheral blood mononuclear cell Toll-like receptor 2/3 expression 
      and reactivity to their ligands in a cohort of patients with wet age-related 
      macular degeneration.
PG  - 1826-33
AB  - PURPOSE: To investigate Toll-like receptor (TLR) expression and reactivity in 
      patients with the wet form age-related macular degeneration (AMD). METHODS: Blood 
      samples were collected from 25 patients with wet AMD and 25 age-matched healthy 
      controls. Peripheral blood mononuclear cells (PBMCs) were isolated with 
      Ficoll-Hypaque density gradient centrifugation. Expression of TLR1 to TLR10 mRNAs 
      in PBMCs from 15 patients with wet AMD and 15 controls was assessed with 
      real-time PCR. TLR2 and TLR3 protein levels in PBMCs from six patients with wet 
      AMD and six controls were measured with flow cytometry. After PBMCs were 
      stimulated with peptidoglycan (PGN) and poly(I:C), the specific ligands of TLR2 
      and TLR3, cytokines interleukin-6 (IL-6), IL-8, VEGF, and monocyte 
      chemoattractant protein-1 (MCP-1) production in 11 patients with wet AMD and 11 
      controls were assessed. RESULTS: TLR2 and TLR3 mRNA and protein expression in the 
      PBMCs of the patients with wet AMD was significantly higher than that in the 
      controls. However, the difference in TLR1 and TLR4-10 mRNA expression between the 
      two groups was not significant. The PBMCs of the patients with wet AMD produced 
      more IL-6 and IL-8 proteins than the controls in response to PGN, a ligand for 
      TLR2, and more IL-6 protein than the controls in response to poly(I:C), the 
      ligand for TLR3. However, there was no significant difference in vascular 
      endothelial growth factor and monocyte chemoattractant protein-1 production 
      between the wet AMD group and the control group when the PBMCs were stimulated 
      with PGN or poly(I:C). CONCLUSIONS: Our data suggested that upregulation of TLR2 
      and TLR3 may be associated with the pathogenesis of wet AMD.
FAU - Zhu, Yi
AU  - Zhu Y
AD  - Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical 
      University, Chongqing Key Laboratory of Ophthalmology, Chongqing Eye Institute, 
      Chongqing, China.
FAU - Liang, Liang
AU  - Liang L
FAU - Qian, Dan
AU  - Qian D
FAU - Yu, Hongsong
AU  - Yu H
FAU - Yang, Peizeng
AU  - Yang P
FAU - Lei, Bo
AU  - Lei B
FAU - Peng, Hui
AU  - Peng H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130806
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Ligands)
RN  - 0 (RNA, Messenger)
RN  - 0 (TLR2 protein, human)
RN  - 0 (TLR3 protein, human)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 3)
SB  - IM
MH  - Cell Membrane/metabolism
MH  - Cohort Studies
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Ligands
MH  - Macular Degeneration/*blood
MH  - RNA, Messenger/genetics/metabolism
MH  - Toll-Like Receptor 2/*blood/genetics
MH  - Toll-Like Receptor 3/*blood/genetics
PMC - PMC3742118
EDAT- 2013/08/16 06:00
MHDA- 2013/09/26 06:00
PMCR- 2013/01/01
CRDT- 2013/08/16 06:00
PHST- 2013/03/20 00:00 [received]
PHST- 2013/08/03 00:00 [accepted]
PHST- 2013/08/16 06:00 [entrez]
PHST- 2013/08/16 06:00 [pubmed]
PHST- 2013/09/26 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 184 [pii]
PST - epublish
SO  - Mol Vis. 2013 Aug 6;19:1826-33. eCollection 2013.