PMID- 23946810
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 6
IP  - 1
DP  - 2013 Jul
TI  - Expression and significance of hypoxia-inducible factor-1alpha and 
      MDR1/P-glycoprotein in laryngeal carcinoma tissue and hypoxic Hep-2 cells.
PG  - 232-238
AB  - The present study aimed to evaluate the expression of hypoxia-inducible factor-1alpha 
      (HIF-1alpha) and MDR1/P-glycoprotein (P-gp) in human laryngeal squamous cell 
      carcinoma (LSCC) tissues, and also to investigate the regulation of MDR1 gene 
      expression by HIF-1alpha in Hep-2 cells under hypoxic conditions. The expression of 
      HIF-1alpha and MDR1/P-gp in human LSCC tissues was examined using 
      immunohistochemistry. The HIF-1alpha and MDR1 gene expression in the Hep-2 cells was 
      detected using real-time quantitative reverse transcription (QRT)-PCR and western 
      blot analysis under normoxic and hypoxic conditions. In hypoxia, HIF-1alpha 
      expression was inhibited by RNA interference. HIF-1alpha and MDR1/P-gp expression was 
      high in the LSCC tissues and was associated with the clinical stage and lymph 
      node metastasis (P<0.05). HIF-1alpha expression was positively correlated with 
      MDR1/P-gp expression (P<0.01). In the Hep-2 cells, HIF-1alpha and MDR1/P-gp 
      expression significantly increased in response to hypoxia. The inhibition of 
      HIF-1alpha expression synergistically downregulated the expression of the MDR1 gene 
      in hypoxic Hep-2 cells. HIF-1alpha expression is positively correlated with MDR1/P-gp 
      expression in LSCC, and the two proteins may be able to serve as potential 
      biomarkers for predicting the malignant progression and metastasis of LSCC. 
      HIF-1alpha may be critical for the upregulation of MDR1 gene expression induced by 
      hypoxia in Hep-2 cells.
FAU - Xie, Jin
AU  - Xie J
AD  - Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiao Tong University 
      Affiliated First People's Hospital, Shanghai 200080;
FAU - Li, DA-Wei
AU  - Li DW
FAU - Chen, Xin-Wei
AU  - Chen XW
FAU - Wang, Fei
AU  - Wang F
FAU - Dong, Pin
AU  - Dong P
LA  - eng
PT  - Journal Article
DEP - 20130429
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC3742502
OTO - NOTNLM
OT  - hypoxia
OT  - hypoxia inducible factor-1alpha
OT  - laryngeal neoplasms
OT  - multidrug resistance
OT  - p-glycoprotein
EDAT- 2013/08/16 06:00
MHDA- 2013/08/16 06:01
PMCR- 2013/04/29
CRDT- 2013/08/16 06:00
PHST- 2012/12/26 00:00 [received]
PHST- 2013/04/04 00:00 [accepted]
PHST- 2013/08/16 06:00 [entrez]
PHST- 2013/08/16 06:00 [pubmed]
PHST- 2013/08/16 06:01 [medline]
PHST- 2013/04/29 00:00 [pmc-release]
AID - ol-06-01-0232 [pii]
AID - 10.3892/ol.2013.1321 [doi]
PST - ppublish
SO  - Oncol Lett. 2013 Jul;6(1):232-238. doi: 10.3892/ol.2013.1321. Epub 2013 Apr 29.