PMID- 23947943
OWN - NLM
STAT- MEDLINE
DCOM- 20140325
LR  - 20181202
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 14
IP  - 9
DP  - 2013 Sep 9
TI  - Multifunctional polyampholyte hydrogels with fouling resistance and protein 
      conjugation capacity.
PG  - 3112-22
LID - 10.1021/bm4007369 [doi]
AB  - Materials that are resistant to nonspecific protein adsorption are critical in 
      the biomedical community. Specifically, nonfouling implantable biomaterials are 
      necessary to reduce the undesirable, but natural foreign body response. The focus 
      of this investigation is to demonstrate that polyampholyte hydrogels prepared 
      with equimolar quantities of positively charged [2-(acryloyloxy)ethyl] 
      trimethylammonium chloride (TMA) and negatively charged 2-carboxyethyl acrylate 
      (CAA) monomers are a viable solution to this problem. TMA/CAA hydrogels were 
      prepared and their physical and chemical properties were characterized. The 
      fouling resistance of the TMA/CAA hydrogels were assessed at varying cross-linker 
      densities using enzyme-linked immunosorbant assays (ELISAs). The results clearly 
      demonstrate that TMA/CAA hydrogels are resistant to nonspecific protein 
      adsorption. A unique advantage of the fouling resistant TMA/CAA system is that 
      bioactive proteins can be covalently attached to these materials using standard 
      conjugation chemistry. This was demonstrated in this study through a combination 
      of ELISA investigations and short-term cell adhesion assays. The multifunctional 
      properties of the TMA/CAA polyampholyte hydrogels shown in this work clearly 
      demonstrate the potential for these materials for use as tissue regeneration 
      scaffolds for many biomedical applications.
FAU - Schroeder, Megan E
AU  - Schroeder ME
AD  - Department of Chemical Engineering, Columbia, Missouri 65211, United States.
FAU - Zurick, Kevin M
AU  - Zurick KM
FAU - McGrath, Daniel E
AU  - McGrath DE
FAU - Bernards, Matthew T
AU  - Bernards MT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130828
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Acrylates)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Hydrogels)
RN  - 0 (Immobilized Proteins)
RN  - 0 (Polymethacrylic Acids)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 14I47YJ5EY (triethylene glycol dimethacrylate)
RN  - 24615-84-7 (3-acryloyloxypropionic acid)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 9001-32-5 (Fibrinogen)
SB  - IM
MH  - 3T3 Cells
MH  - Acrylates/*chemistry
MH  - Adsorption
MH  - Animals
MH  - Cell Adhesion
MH  - Coated Materials, Biocompatible/chemistry
MH  - Cross-Linking Reagents
MH  - Fibrinogen/*chemistry
MH  - Hydrogels/*chemistry
MH  - Immobilized Proteins/*chemistry
MH  - Materials Testing
MH  - Mice
MH  - Polyethylene Glycols/chemistry
MH  - Polymethacrylic Acids/chemistry
MH  - Quaternary Ammonium Compounds/*chemistry
MH  - Stress, Mechanical
EDAT- 2013/08/21 06:00
MHDA- 2014/03/26 06:00
CRDT- 2013/08/17 06:00
PHST- 2013/08/17 06:00 [entrez]
PHST- 2013/08/21 06:00 [pubmed]
PHST- 2014/03/26 06:00 [medline]
AID - 10.1021/bm4007369 [doi]
PST - ppublish
SO  - Biomacromolecules. 2013 Sep 9;14(9):3112-22. doi: 10.1021/bm4007369. Epub 2013 
      Aug 28.