PMID- 23948641
OWN - NLM
STAT- MEDLINE
DCOM- 20140611
LR  - 20211108
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 252
DP  - 2013 Nov 12
TI  - AQP1 expression alterations affect morphology and water transport in Schwann 
      cells and hypoxia-induced up-regulation of AQP1 occurs in a HIF-1alpha-dependent 
      manner.
PG  - 68-79
LID - S0306-4522(13)00684-2 [pii]
LID - 10.1016/j.neuroscience.2013.08.006 [doi]
AB  - Aquaporin-1 (AQP1) is the principle water channel in the peripheral nervous 
      system (PNS) and is specifically localized to Schwann cells in the PNS. However, 
      the pathophysiological role of AQP1 in peripheral nerves is poorly understood. 
      Here, we utilized RNA interference by lentiviral transduction to specifically 
      down-regulate AQP1 expression and a lentiviral overexpression protocol to 
      up-regulate AQP1 expression, in primary Schwann cell cultures. AQP1 gene 
      silencing resulted in a cell shrinkage phenotype, while AQP1 gene overexpression 
      caused a cell swelling phenotype, as validated by cell volume determinations. 
      Secondly, we utilized an in vitro hypoxia model in Schwann cells to mimic in vivo 
      facial nerve injury. We demonstrated that AQP1 expression was induced within 8h 
      following hypoxia injury in vitro, and that AQP1 knockdown (KD) caused the cells 
      to resist edema following hypoxia. Finally, we investigated the hypoxic 
      regulation of the AQP1 gene, as well as the involvement of Hypoxia-inducible 
      factor-1alpha (HIF-1alpha) in AQP1 modulation and we found that KD of HIF-1alpha decreased 
      hypoxia-dependent induction of endogenous AQP1 expression at both the mRNA and 
      protein levels. Taken together, these results indicate that (1) AQP1 is an 
      important factor responsible for the fast water transport of cultured Schwann 
      cells and is involved in cell plasticity; (2) AQP1 alterations may be a primary 
      factor in hypoxia-induced peripheral nerve edema; (3) HIF-1alpha participates in the 
      hypoxic induction of the AQP1 gene; (4) AQP1 inhibition might provide a new 
      therapeutic alternative for the treatment of some forms of peripheral nerve 
      edema.
CI  - Crown Copyright (c) 2013. Published by Elsevier Ltd. All rights reserved.
FAU - Zhang, J
AU  - Zhang J
AD  - Department of Plastic Surgery, Changzheng Hospital, Second Military Medical 
      University, Shanghai 200003, China; Department of Plastic Surgery, The First 
      Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China.
FAU - Xiong, Y
AU  - Xiong Y
FAU - Lu, L X
AU  - Lu LX
FAU - Wang, H
AU  - Wang H
FAU - Zhang, Y F
AU  - Zhang YF
FAU - Fang, F
AU  - Fang F
FAU - Song, Y L
AU  - Song YL
FAU - Jiang, H
AU  - Jiang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130812
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Aqp1 protein, mouse)
RN  - 0 (Hif1a protein, mouse)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 059QF0KO0R (Water)
RN  - 146410-94-8 (Aquaporin 1)
SB  - IM
EIN - Neuroscience. 2014 Feb 28;260:276
MH  - Animals
MH  - Aquaporin 1/genetics/*metabolism
MH  - Blotting, Western
MH  - Cell Hypoxia/physiology
MH  - Edema/genetics/metabolism
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation
MH  - Gene Knockdown Techniques
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - RNA Interference
MH  - Real-Time Polymerase Chain Reaction
MH  - Schwann Cells/cytology/*metabolism
MH  - Transfection
MH  - Up-Regulation
MH  - Water/*metabolism
OTO - NOTNLM
OT  - AQP1
OT  - AQP1-shRNA
OT  - AQP1-specific shRNA vectors
OT  - AQPs
OT  - CoCl(2)
OT  - GFAP
OT  - HIF-1alpha
OT  - HIFs
OT  - KD
OT  - OMG
OT  - PBS
OT  - PCR
OT  - PNS
OT  - Schwann cells
OT  - VEGF
OT  - [3H]-O-methylglucose
OT  - aquaporins
OT  - cobalt(II) chloride
OT  - glial fibrillary acidic protein
OT  - hypoxia-inducible factor-1alpha
OT  - hypoxia-inducible factors
OT  - knockdown
OT  - lenti-AQP1
OT  - lentivirus-AQP1
OT  - nerve edema
OT  - peripheral nerve system
OT  - peripheral nervous system
OT  - phosphate-buffered saline
OT  - polymerase chain reaction
OT  - scr-shRNA
OT  - scramble-shRNA vector
OT  - shRNA
OT  - short hairpin RNA
OT  - vascular endothelial growth factor
OT  - water transport
EDAT- 2013/08/21 06:00
MHDA- 2014/06/12 06:00
CRDT- 2013/08/17 06:00
PHST- 2013/04/20 00:00 [received]
PHST- 2013/07/05 00:00 [revised]
PHST- 2013/08/03 00:00 [accepted]
PHST- 2013/08/17 06:00 [entrez]
PHST- 2013/08/21 06:00 [pubmed]
PHST- 2014/06/12 06:00 [medline]
AID - S0306-4522(13)00684-2 [pii]
AID - 10.1016/j.neuroscience.2013.08.006 [doi]
PST - ppublish
SO  - Neuroscience. 2013 Nov 12;252:68-79. doi: 10.1016/j.neuroscience.2013.08.006. 
      Epub 2013 Aug 12.