PMID- 23949303
OWN - NLM
STAT- MEDLINE
DCOM- 20140318
LR  - 20211203
IS  - 1573-904X (Electronic)
IS  - 0724-8741 (Print)
IS  - 0724-8741 (Linking)
VI  - 30
IP  - 9
DP  - 2013 Sep
TI  - Effect of caloric restriction and AMPK activation on hepatic nuclear receptor, 
      biotransformation enzyme, and transporter expression in lean and obese mice.
PG  - 2232-47
LID - 10.1007/s11095-013-1140-2 [doi]
AB  - PURPOSE: Fatty liver alters liver transporter expression. Caloric restriction 
      (CR), the recommended therapy to reverse fatty liver, increases Sirtuin1 
      deacetylase activity in liver. This study evaluated whether CR and CR mimetics 
      reversed obesity-induced transporter expression in liver and hepatocytes. 
      METHODS: mRNA and protein expression was determined in adult lean (lean) and 
      leptin-deficient obese (OB) mice fed ad libitum or placed on 40% (kCal) reduced 
      diet. Hepatocytes were isolated from lean and OB mice, treated with AMP Kinase 
      activators, and gene expression was determined. RESULTS: CR decreased Oatp1a1, 
      Oatp1b2, and Abcb11 mRNA expression in lean, but not OB mice. CR increased Abcc2 
      mRNA OB livers, whereas protein expression increased in both genotypes. CR 
      increased Abcc3 protein expression increased in OB livers. CR did not alter 
      Abcc1, 4 and 5 mRNA expression in lean mice but decreased expression in livers of 
      OB mice. CR increased Abcc4 protein in lean, but not OB mice. CONCLUSIONS: CR 
      restriction reversed the expression of some, but not all transporters in livers 
      of OB mice. Overall, these data indicate a potential for CR to restore some 
      hepatic transporter changes in OB mice, but suggest a functional leptin axis is 
      needed for reversal of expression for some transporters.
FAU - Kulkarni, Supriya R
AU  - Kulkarni SR
AD  - Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, 7 
      Greenhouse Road, Kingston, Rhode Island, 02881, USA.
FAU - Xu, Jialin
AU  - Xu J
FAU - Donepudi, Ajay C
AU  - Donepudi AC
FAU - Wei, Wei
AU  - Wei W
FAU - Slitt, Angela L
AU  - Slitt AL
LA  - eng
GR  - P20 GM103430/GM/NIGMS NIH HHS/United States
GR  - 5K22ES013782/ES/NIEHS NIH HHS/United States
GR  - K22 ES013782/ES/NIEHS NIH HHS/United States
GR  - R01 ES016042/ES/NIEHS NIH HHS/United States
GR  - P20RR016457/RR/NCRR NIH HHS/United States
GR  - P20 RR016457/RR/NCRR NIH HHS/United States
GR  - 4R01ES016042/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130816
PL  - United States
TA  - Pharm Res
JT  - Pharmaceutical research
JID - 8406521
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 11)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Abcb11 protein, mouse)
RN  - 0 (Lipids)
RN  - 0 (Liver-Specific Organic Anion Transporter 1)
RN  - 0 (Multidrug Resistance-Associated Protein 2)
RN  - 0 (Multidrug Resistance-Associated Proteins)
RN  - 0 (Oatp1a1 protein, mouse)
RN  - 0 (Organic Anion Transporters, Sodium-Independent)
RN  - 0 (Organic Cation Transport Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Slco1b2 protein, mouse)
RN  - 1YV0492L5Z (multidrug resistance-associated protein 3)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 11
MH  - ATP-Binding Cassette Transporters/genetics
MH  - Animals
MH  - Body Weight
MH  - *Caloric Restriction
MH  - Cells, Cultured
MH  - Enzyme Activation
MH  - *Gene Expression Regulation
MH  - Lipids/analysis
MH  - Liver/*metabolism/pathology
MH  - Liver-Specific Organic Anion Transporter 1
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
MH  - Multidrug Resistance-Associated Protein 2
MH  - Multidrug Resistance-Associated Proteins/genetics
MH  - Obesity/*diet therapy/enzymology/*genetics/pathology
MH  - Organic Anion Transporters, Sodium-Independent/genetics
MH  - Organic Cation Transport Proteins/genetics
MH  - RNA, Messenger/genetics
MH  - Receptors, Cytoplasmic and Nuclear/genetics
PMC - PMC4225720
MID - NIHMS516336
EDAT- 2013/08/21 06:00
MHDA- 2014/03/19 06:00
PMCR- 2014/11/10
CRDT- 2013/08/17 06:00
PHST- 2013/01/08 00:00 [received]
PHST- 2013/07/01 00:00 [accepted]
PHST- 2013/08/17 06:00 [entrez]
PHST- 2013/08/21 06:00 [pubmed]
PHST- 2014/03/19 06:00 [medline]
PHST- 2014/11/10 00:00 [pmc-release]
AID - 10.1007/s11095-013-1140-2 [doi]
PST - ppublish
SO  - Pharm Res. 2013 Sep;30(9):2232-47. doi: 10.1007/s11095-013-1140-2. Epub 2013 Aug 
      16.