PMID- 23954596
OWN - NLM
STAT- MEDLINE
DCOM- 20140327
LR  - 20130911
IS  - 1096-0279 (Electronic)
IS  - 1046-5928 (Linking)
VI  - 91
IP  - 2
DP  - 2013 Oct
TI  - The crystal structure and biochemical properties of DHBPS from Streptococcus 
      pneumoniae, a potential anti-infective target for Gram-positive bacteria.
PG  - 161-8
LID - S1046-5928(13)00133-2 [pii]
LID - 10.1016/j.pep.2013.07.007 [doi]
AB  - The enzymes involved in riboflavin biosynthesis are considered to be potential 
      anti-bacterial drug targets because these proteins are essential in bacterial 
      pathogens but are absent in humans. 3,4-dihydroxy-2-butanone-4-phosphate synthase 
      (DHBPS) is one of the key enzymes in the biosynthesis of riboflavin. DHBPS 
      catalyzes the conversion of ribulose-5-phosphate (Ru5P) to 
      3,4-Dihydroxy-2-butanone-4-phosphate (DHBP) and formate. The purified SpDHBPS 
      enzyme, in the presence of Mg(2+) ion, catalyzed the conversion of Ru5P to DHBP 
      at a rate of 109nmolmin(-1)mg(-1) with an apparent Km value of 181muM at 37 degrees C. 
      Surprisingly, our experiments first revealed that DHBPS showed activity in the 
      presence of the trivalent metal ion, Fe(3+). Furthermore, we determined the 
      crystal structure of DHBPS from Gram-positive bacteria, Streptococcus pneumoniae, 
      with 2.0A resolution. The overall architecture of SpDHBPS was similar to its 
      homologs, which comprise one beta-sheet (five-stranded) and eight alpha-helices, 
      adopting a three-layered alpha-beta-alpha sandwich fold. Similar to the homologs, 
      gel-filtration experiments verified that the enzyme was arranged as a dimer. 
      Although the overall fold of DHBPS was similar, the significant structural 
      differences between the species at the active site region may be utilized to 
      develop antibacterial agents that are species-specific.
CI  - Crown Copyright (c) 2013. Published by Elsevier Inc. All rights reserved.
FAU - Li, Jin
AU  - Li J
AD  - Key Laboratory of Molecular Biology on Infectious Disease, Chongqing Medical 
      University, YiXueYuanlu-1, Chongqing 400016, People's Republic of China; 
      Department of Laboratory Medicine, Chongqing Medical University, YiXueYuanlu-1, 
      Chongqing 400016, People's Republic of China.
FAU - Hua, Zhou
AU  - Hua Z
FAU - Miao, Luo
AU  - Miao L
FAU - Jian, Tang
AU  - Jian T
FAU - Wei, Yang
AU  - Wei Y
FAU - Shasha, Zhao
AU  - Shasha Z
FAU - Shaocheng, Zhang
AU  - Shaocheng Z
FAU - Zhen, Guo
AU  - Zhen G
FAU - Hongpeng, Zhang
AU  - Hongpeng Z
FAU - Ailong, Huang
AU  - Ailong H
FAU - Deqiang, Wang
AU  - Deqiang W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130815
PL  - United States
TA  - Protein Expr Purif
JT  - Protein expression and purification
JID - 9101496
RN  - 0 (Bacterial Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 5.4.- (Intramolecular Transferases)
RN  - EC 5.4.- (L-3,4-dihydroxy-2-butanone-4-phosphate synthase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Bacterial Proteins/*chemistry/genetics/isolation & purification/metabolism
MH  - Catalytic Domain
MH  - Drug Delivery Systems
MH  - Enzyme Stability
MH  - Escherichia coli/genetics/metabolism
MH  - Intramolecular Transferases/*chemistry/genetics/isolation & 
      purification/metabolism
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Protein Conformation
MH  - Protein Multimerization
MH  - Recombinant Fusion Proteins/chemistry/genetics/isolation & 
      purification/metabolism
MH  - Sequence Alignment
MH  - Streptococcus pneumoniae/*enzymology/genetics
OTO - NOTNLM
OT  - 3,4-Dihydroxy-2-butanone-4-phosphate synthase
OT  - 3,4-dihydroxy-2-butanone-4-phosphate
OT  - 3,4-dihydroxy-2-butanone-4-phosphate synthase
OT  - 3,4-dihydroxy-2-butanone-4-phosphate synthase of Streptococcus pneumonia
OT  - Crystal structure
OT  - DHBP
OT  - DHBPS
OT  - DMSO
OT  - Enzyme analysis
OT  - PCR
OT  - PDB
OT  - Ru5P
OT  - S. pneumonia
OT  - SpDHBPS
OT  - Streptococcus pneumonia
OT  - Streptococcus pneumoniae
OT  - dimethyl sulfoxide
OT  - polymerase chain reaction
OT  - protein data bank
OT  - ribulose-5-phosphate
EDAT- 2013/08/21 06:00
MHDA- 2014/03/29 06:00
CRDT- 2013/08/20 06:00
PHST- 2013/04/26 00:00 [received]
PHST- 2013/07/09 00:00 [revised]
PHST- 2013/07/11 00:00 [accepted]
PHST- 2013/08/20 06:00 [entrez]
PHST- 2013/08/21 06:00 [pubmed]
PHST- 2014/03/29 06:00 [medline]
AID - S1046-5928(13)00133-2 [pii]
AID - 10.1016/j.pep.2013.07.007 [doi]
PST - ppublish
SO  - Protein Expr Purif. 2013 Oct;91(2):161-8. doi: 10.1016/j.pep.2013.07.007. Epub 
      2013 Aug 15.