PMID- 23955414
OWN - NLM
STAT- MEDLINE
DCOM- 20160422
LR  - 20181202
IS  - 0794-859X (Print)
IS  - 0794-859X (Linking)
VI  - 28
IP  - 1
DP  - 2013 Jun 30
TI  - Anti-diabetic property of Methanol extract of Musa sapientum leaves and its 
      fractions in alloxan-induced diabetic rats.
PG  - 91-7
AB  - Diabetes mellitus is a metabolic disorder resulting from necrosis of beta-cell and 
      insulin resistance at the cellular level. Musa sapientum has been shown to 
      possess anti-diabetic properties, however, the mechanism of its action is 
      unknown. The effect of Methanolic extract of Musa sapientum leaves (MEMSL) and 
      its fractions were assessed for in vitro inhibitory activity of alpha-amylase enzyme, 
      in vivo hypoglycemic properties and liver glycogen content in alloxan-induced 
      diabetic rats. Dried plant powder of Musa sapientum was successively extracted 
      using n-hexane, ethyl acetate, dichloromethane and methanol respectively. The 
      filtrate obtained was evaporated using rotary evaporator and the extract was 
      stored at 4 degrees C until use. The methanolic extract obtained was further fractionated 
      using column chromatography. In vitro alpha amylase inhibitory activity of the 
      methanolic extract at different doses (2.5mg/ml, 5mg/ml, 10mg/ml, 25mg/ml and 
      50mg/ml) and column fractions (100ug/ml) were assessed and compared with that of 
      acarbose (5mg/ml), a standard oral alpha-amylase inhibitor. Hypoglycemic activity and 
      liver glycogen content was studied using alloxan -induced diabetic male rats 
      treated with MEMSL (250mg/kg and 500mg/kg), column fractions F2 and F5 (100mug/kg) 
      for 14 days respectively. Results obtained showed a dose -dependent increase in 
      alpha-amylase inhibitory activity of the methanolic extract at 5, 10, 25 and 50mg/ml 
      exhibiting 29%, 61%, and 72% and 80% inhibitory activities respectively. Column 
      fractions 2 and 5 showed the highest alpha-amylase inhibitory activity of 79% and 74% 
      respectively. The MEMSL at 250mg/kg and 500mg/kg exhibited 66% and 59% 
      hypoglycemic activities respectively compared with diabetic controls. Fractions 2 
      and 5 showed 48% and 75% reduction in blood glucose level respectively. Liver 
      glycogen in diabetic animals treated with MEMSL (250mg/kg and 500mg/kg), F2 and 
      F5 were significantly increased (5.5+/-0.5, 5.9+/-0.7, 3.6+/-0.5, 8.0+/-0.4 mg/100gwt. 
      liver) compared with Diabetic controls (1.2+/-0.3 mg/100gwt. liver) respectively 
      suggesting an increase in glucose storage or reduction in glycogen breakdown. It 
      seems possible that the anti-diabetic properties in the leaf extract of Musa 
      sapientum and its fractions maybe due to the inhibition of alpha-amylase, increased 
      storage of glucose as glycogen in the liver and/or reduced breakdown of liver 
      glycogen stores.
FAU - Adewoye, E O
AU  - Adewoye EO
AD  - Department of Physiology, College of Medicine, University of Ibadan, Ibadan. 
      elolade@yahoo.com.
FAU - Ige, A O
AU  - Ige AO
LA  - eng
PT  - Journal Article
DEP - 20130630
PL  - Nigeria
TA  - Niger J Physiol Sci
JT  - Nigerian journal of physiological sciences : official publication of the 
      Physiological Society of Nigeria
JID - 8811109
RN  - 0 (Blood Glucose)
RN  - 0 (Plant Extracts)
RN  - 6SW5YHA5NG (Alloxan)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - *Alloxan
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Diabetes Mellitus, Experimental/drug therapy
MH  - Methanol/chemistry
MH  - *Musa/chemistry
MH  - Plant Extracts/pharmacology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2013/08/21 06:00
MHDA- 2016/04/23 06:00
CRDT- 2013/08/20 06:00
PHST- 2013/08/10 00:00 [received]
PHST- 2013/08/10 00:00 [accepted]
PHST- 2013/08/20 06:00 [entrez]
PHST- 2013/08/21 06:00 [pubmed]
PHST- 2016/04/23 06:00 [medline]
PST - epublish
SO  - Niger J Physiol Sci. 2013 Jun 30;28(1):91-7.