PMID- 23955606
OWN - NLM
STAT- MEDLINE
DCOM- 20140304
LR  - 20211021
IS  - 1941-7225 (Electronic)
IS  - 0895-7061 (Print)
IS  - 0895-7061 (Linking)
VI  - 26
IP  - 12
DP  - 2013 Dec
TI  - Euglycemic hyperinsulinemia increases blood pressure in pregnant rats independent 
      of placental antiangiogenic and inflammatory factors.
PG  - 1445-51
LID - 10.1093/ajh/hpt137 [doi]
AB  - BACKGROUND: Although pregnancies associated with hyperinsulinemia and altered 
      placental angiogenic and inflammatory factors are at increased risk for 
      developing preeclampsia, the effects of euglycemic hyperinsulinemia on placental 
      factors and blood pressure regulation during pregnancy are unclear. We 
      hypothesized that chronic hyperinsulinemia results in increased placental soluble 
      fms-like tyrosine kinase 1(sFlt-1) and tumor necrosis factor alpha (TNF- alpha) levels 
      and hypertension in pregnant rats. METHODS: On gestational day (GD) 14, 
      Sprague-Dawley rats were assigned as normal pregnant or pregnant + insulin. 
      Insulin was infused subcutaneously by osmotic minipump for 5 days at a dose of 
      1.5 mU/kg/min. Those rats receiving insulin were supplemented with 20% glucose in 
      drinking water to maintain euglycemia. On GD 19, mean arterial pressure (MAP) and 
      heart rate (HR) were assessed in conscious rats by indwelling carotid catheters, 
      followed by collections of blood, placentas, and fetuses. In addition to 
      placental sFlt-1 and TNF-alpha levels, circulating insulin, glucose, leptin, 
      cholesterol, triglyceride, and free fatty acid concentrations were measured. 
      RESULTS: MAP was higher in pregnant + insulin vs. normal pregnant rats; however, 
      HR was similar between groups. Although litter size and placental weight were 
      comparable, fetuses from pregnant + insulin rats were heavier. Importantly, 
      circulating insulin concentration was elevated in the pregnant + insulin group, 
      with no change in glucose level. Moreover, circulating leptin, cholesterol, 
      triglyceride, and free fatty acid concentrations were increased in the pregnant + 
      insulin group. There were no differences in placental sFlt-1 and TNF-alpha 
      concentrations between groups. CONCLUSIONS: In summary, sustained euglycemic 
      hyperinsulinemia, comparable with insulin levels in preeclamptic women, can raise 
      blood pressure in pregnancy independent of recognized placental factors 
      associated with preeclampsia.
FAU - Palei, Ana C
AU  - Palei AC
AD  - Department of Physiology and Biophysics, University of Mississippi Medical 
      Center, Jackson, Mississippi.
FAU - Spradley, Frank T
AU  - Spradley FT
FAU - Granger, Joey P
AU  - Granger JP
LA  - eng
GR  - P01 HL051971/HL/NHLBI NIH HHS/United States
GR  - T32 HL105324/HL/NHLBI NIH HHS/United States
GR  - 1T32HL105324/HL/NHLBI NIH HHS/United States
GR  - HL051971/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130816
PL  - United States
TA  - Am J Hypertens
JT  - American journal of hypertension
JID - 8803676
RN  - 0 (Biomarkers)
RN  - 0 (Insulin)
RN  - 0 (Leptin)
RN  - 0 (Triglycerides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-1)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Biomarkers/*blood
MH  - Blood Pressure/*drug effects
MH  - Cholesterol/blood
MH  - Disease Models, Animal
MH  - Female
MH  - Glucose/metabolism
MH  - Heart Rate/drug effects
MH  - Hyperinsulinism/*blood
MH  - Hypertension/*blood
MH  - Insulin/blood
MH  - Leptin/blood
MH  - Placenta/*physiopathology
MH  - Pre-Eclampsia/blood/physiopathology
MH  - Pregnancy
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Triglycerides/blood
MH  - Tumor Necrosis Factor-alpha/blood
MH  - Vascular Endothelial Growth Factor Receptor-1/blood
PMC - PMC3816628
OTO - NOTNLM
OT  - blood pressure
OT  - hyperinsulinemia
OT  - hypertension
OT  - preeclampsia.
OT  - pregnancy
EDAT- 2013/08/21 06:00
MHDA- 2014/03/05 06:00
PMCR- 2014/12/01
CRDT- 2013/08/20 06:00
PHST- 2013/08/20 06:00 [entrez]
PHST- 2013/08/21 06:00 [pubmed]
PHST- 2014/03/05 06:00 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - hpt137 [pii]
AID - 10.1093/ajh/hpt137 [doi]
PST - ppublish
SO  - Am J Hypertens. 2013 Dec;26(12):1445-51. doi: 10.1093/ajh/hpt137. Epub 2013 Aug 
      16.