PMID- 23956388
OWN - NLM
STAT- MEDLINE
DCOM- 20140602
LR  - 20211021
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 79
IP  - 20
DP  - 2013 Oct
TI  - Methanosarcinaceae and acetate-oxidizing pathways dominate in high-rate 
      thermophilic anaerobic digestion of waste-activated sludge.
PG  - 6491-500
LID - 10.1128/AEM.01730-13 [doi]
AB  - This study investigated the process of high-rate, high-temperature methanogenesis 
      to enable very-high-volume loading during anaerobic digestion of waste-activated 
      sludge. Reducing the hydraulic retention time (HRT) from 15 to 20 days in 
      mesophilic digestion down to 3 days was achievable at a thermophilic temperature 
      (55 degrees C) with stable digester performance and methanogenic activity. A volatile 
      solids (VS) destruction efficiency of 33 to 35% was achieved on waste-activated 
      sludge, comparable to that obtained via mesophilic processes with low organic 
      acid levels (<200 mg/liter chemical oxygen demand [COD]). Methane yield (VS 
      basis) was 150 to 180 liters of CH4/kg of VS(added). According to 16S rRNA 
      pyrotag sequencing and fluorescence in situ hybridization (FISH), the 
      methanogenic community was dominated by members of the Methanosarcinaceae, which 
      have a high level of metabolic capability, including acetoclastic and 
      hydrogenotrophic methanogenesis. Loss of function at an HRT of 2 days was 
      accompanied by a loss of the methanogens, according to pyrotag sequencing. The 
      two acetate conversion pathways, namely, acetoclastic methanogenesis and 
      syntrophic acetate oxidation, were quantified by stable carbon isotope ratio mass 
      spectrometry. The results showed that the majority of methane was generated by 
      nonacetoclastic pathways, both in the reactors and in off-line batch tests, 
      confirming that syntrophic acetate oxidation is a key pathway at elevated 
      temperatures. The proportion of methane due to acetate cleavage increased later 
      in the batch, and it is likely that stable oxidation in the continuous reactor 
      was maintained by application of the consistently low retention time.
FAU - Ho, Dang P
AU  - Ho DP
AD  - Advanced Water Management Centre, The University of Queensland, St Lucia, 
      Queensland, Australia.
FAU - Jensen, Paul D
AU  - Jensen PD
FAU - Batstone, Damien J
AU  - Batstone DJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130816
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Acetates)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Acetates/*metabolism
MH  - Anaerobiosis
MH  - *Biota
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Methanosarcinaceae/classification/*isolation & purification/physiology
MH  - Oxidation-Reduction
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - Temperature
PMC - PMC3811223
EDAT- 2013/08/21 06:00
MHDA- 2014/06/03 06:00
PMCR- 2014/04/01
CRDT- 2013/08/20 06:00
PHST- 2013/08/20 06:00 [entrez]
PHST- 2013/08/21 06:00 [pubmed]
PHST- 2014/06/03 06:00 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - AEM.01730-13 [pii]
AID - 01730-13 [pii]
AID - 10.1128/AEM.01730-13 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2013 Oct;79(20):6491-500. doi: 10.1128/AEM.01730-13. Epub 
      2013 Aug 16.