PMID- 23958436
OWN - NLM
STAT- MEDLINE
DCOM- 20131210
LR  - 20211021
IS  - 1095-564X (Electronic)
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 382
IP  - 2
DP  - 2013 Oct 15
TI  - Enteric neural crest-derived cells promote their migration by modifying their 
      microenvironment through tenascin-C production.
PG  - 446-56
LID - S0012-1606(13)00418-1 [pii]
LID - 10.1016/j.ydbio.2013.08.006 [doi]
AB  - The enteric nervous system (ENS) is derived from vagal and sacral neural crest 
      cells that migrate, proliferate, and differentiate into enteric neurons and glia 
      within the gut wall. The mechanisms regulating enteric neural crest-derived cell 
      (ENCC) migration are poorly characterized despite the importance of this process 
      in gut formation and function. Characterization of genes involved in ENCC 
      migration is essential to understand ENS development and could provide targets 
      for treatment of human ENS disorders. We identified the extracellular matrix 
      glycoprotein tenascin-C (TNC) as an important regulator of ENCC development. We 
      find TNC dynamically expressed during avian gut development. It is absent from 
      the cecal region just prior to ENCC arrival, but becomes strongly expressed 
      around ENCCs as they enter the ceca and hindgut. In aganglionic hindguts, TNC 
      expression is strong throughout the outer mesenchyme, but is absent from the 
      submucosal region, supporting the presence of both ENCC-dependent and independent 
      expression within the gut wall. Using rat-chick coelomic grafts, neural tube 
      cultures, and gut explants, we show that ENCCs produce TNC and that this ECM 
      protein promotes their migration. Interestingly, only vagal neural crest-derived 
      ENCCs express TNC, whereas sacral neural crest-derived cells do not. These 
      results demonstrate that vagal crest-derived ENCCs actively modify their 
      microenvironment through TNC expression and thereby help to regulate their own 
      migration.
CI  - (c) 2013 Elsevier Inc. All rights reserved.
FAU - Akbareian, Sophia E
AU  - Akbareian SE
AD  - Department of Pediatric Surgery, Massachusetts General Hospital, Harvard Medical 
      School, Warren 1153, Boston, MA 02114, USA.
FAU - Nagy, Nandor
AU  - Nagy N
FAU - Steiger, Casey E
AU  - Steiger CE
FAU - Mably, John D
AU  - Mably JD
FAU - Miller, Sarah A
AU  - Miller SA
FAU - Hotta, Ryo
AU  - Hotta R
FAU - Molnar, David
AU  - Molnar D
FAU - Goldstein, Allan M
AU  - Goldstein AM
LA  - eng
GR  - P30 DK034854/DK/NIDDK NIH HHS/United States
GR  - R01 DK080914/DK/NIDDK NIH HHS/United States
GR  - R01DK080914/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130816
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - 0 (Tenascin)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Cell Movement/*genetics
MH  - Chick Embryo
MH  - Embryo, Mammalian
MH  - Enteric Nervous System/cytology/embryology
MH  - Neural Crest/cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tenascin/*biosynthesis
PMC - PMC3800188
MID - NIHMS516780
OTO - NOTNLM
OT  - Enteric nervous system
OT  - Extracellular matrix
OT  - Hirschsprung disease
OT  - Neural crest cells
OT  - Tenascin-C
EDAT- 2013/08/21 06:00
MHDA- 2013/12/16 06:00
PMCR- 2014/10/15
CRDT- 2013/08/21 06:00
PHST- 2013/03/03 00:00 [received]
PHST- 2013/08/06 00:00 [revised]
PHST- 2013/08/08 00:00 [accepted]
PHST- 2013/08/21 06:00 [entrez]
PHST- 2013/08/21 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2014/10/15 00:00 [pmc-release]
AID - S0012-1606(13)00418-1 [pii]
AID - 10.1016/j.ydbio.2013.08.006 [doi]
PST - ppublish
SO  - Dev Biol. 2013 Oct 15;382(2):446-56. doi: 10.1016/j.ydbio.2013.08.006. Epub 2013 
      Aug 16.