PMID- 23963795
OWN - NLM
STAT- MEDLINE
DCOM- 20140502
LR  - 20130930
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 91
IP  - 11
DP  - 2013 Nov
TI  - Axonal fasciculation and the role of polysialic acid-neural cell adhesion 
      molecule in rat cortical neurons.
PG  - 1408-18
LID - 10.1002/jnr.23268 [doi]
AB  - Axonal fasciculation is a mechanism deployed by growing axons to reach their 
      targets during development of the nervous system. Published data have suggested 
      the involvement of neuronal cell adhesion molecules (NCAM) in axonal 
      fasciculation. We have characterized the formation of axonal fascicles in 
      serum-free, primary cultures of cortical neurons from embryonic rat brains. 
      Unlike the published data, axonal fascicles in our system have a unique 
      morphology: they are waveform, are rarely thicker than 20 mum, and can reach up to 
      several millimeters in length. We observed an age and time dependence in the 
      formation of fascicles. They formed only in cultures from embryonic day 15-17 
      brain and only between 4 days in vitro (DIV) and 11 DIV. Electron microscopy 
      showed that the fascicles consisted of mostly axonal processes. 
      Immunocytochemical staining confirmed that the fascicles were positive for the 
      66-kDa neurofilament protein, NF66, but they contained few, if any, 
      microtubule-associated protein-2-positive or glial fibrillary acidic 
      protein-positive processes. Polysialic acids appeared to be critical in the 
      formation of fascicles. Neuraminidase treatment prevented the formation of 
      fascicles when added before 5 DIV. Addition of a specific inhibitor blocked the 
      effect of neuraminidase. The cortical neurons in our model shared several 
      important features with axon fasciculation in vivo and may provide a unique 
      system for studying the molecular mechanisms involved in the formation of axonal 
      tracts in the brain.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Wakade, Chandramohan G
AU  - Wakade CG
AD  - Department of Physical Therapy, Georgia Regents University, Augusta, Georgia; 
      Institute of Molecular Medicine and Genetics, Georgia Regents University, 
      Augusta, Georgia.
FAU - Mehta, Shyamal H
AU  - Mehta SH
FAU - Maeda, Manebu
AU  - Maeda M
FAU - Webb, R Clinton
AU  - Webb RC
FAU - Chiu, Fung-Chow
AU  - Chiu FC
LA  - eng
PT  - Journal Article
DEP - 20130821
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Neural Cell Adhesion Molecule L1)
RN  - 0 (Sialic Acids)
RN  - 0 (polysialyl neural cell adhesion molecule)
SB  - IM
MH  - Animals
MH  - Axons/metabolism/*ultrastructure
MH  - Cells, Cultured
MH  - Cerebral Cortex/*embryology/metabolism/ultrastructure
MH  - Embryo, Mammalian
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Electron, Transmission
MH  - Neural Cell Adhesion Molecule L1/*metabolism
MH  - Neurogenesis/*physiology
MH  - Rats
MH  - Sialic Acids/*metabolism
OTO - NOTNLM
OT  - NCAM
OT  - axonal fasciculation
OT  - cortical neurons
OT  - neurofilaments
OT  - polysialic acid
EDAT- 2013/08/22 06:00
MHDA- 2014/05/03 06:00
CRDT- 2013/08/22 06:00
PHST- 2013/04/23 00:00 [received]
PHST- 2013/05/20 00:00 [revised]
PHST- 2013/05/23 00:00 [accepted]
PHST- 2013/08/22 06:00 [entrez]
PHST- 2013/08/22 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
AID - 10.1002/jnr.23268 [doi]
PST - ppublish
SO  - J Neurosci Res. 2013 Nov;91(11):1408-18. doi: 10.1002/jnr.23268. Epub 2013 Aug 
      21.