PMID- 23963866
OWN - NLM
STAT- MEDLINE
DCOM- 20140227
LR  - 20211021
IS  - 1573-2959 (Electronic)
IS  - 0167-6369 (Linking)
VI  - 186
IP  - 1
DP  - 2014 Jan
TI  - Aerobiological, clinical, and immunobiochemical studies on Alstonia scholaris 
      pollen from Eastern India.
PG  - 457-67
LID - 10.1007/s10661-013-3390-1 [doi]
AB  - Alstonia scholaris or Indian devil tree is a common, evergreen, tropical tree of 
      the Apocynaceae family. The objectives of this study were (a) to observe the 
      seasonal variation of A. scholaris pollen in the atmosphere of an industrial and 
      rural area of West Bengal, India by conducting a 2-year aerobiological survey 
      with a Burkard personal volumetric sampler, (b) to study its allergenicity in the 
      local population by in vivo (skin-prick test) and in vitro tests (enzyme-linked 
      immunosorbent assay and dot blotting), (c) to identify the immunoglobulin E 
      (IgE)-binding proteins present in the pollen extract (sodium dodecyl 
      sulphate-polyacrylamide gel electrophoresis and immunoblotting), (d) to study its 
      chemical composition. A. scholaris pollen were present in the air from September 
      until November. They contained 14.3 % carbohydrate, 9.2 % lipid, and 4.3 % 
      protein. Among 140 respiratory allergic local patients, 28.57 % showed positive 
      skin reaction to A. scholaris pollen extract. Twelve protein bands in the range 
      of 94.4-13.3 kDa were observed in the pollen extract. Seven IgE-binding proteins 
      were found. Among them, one component of 29.9 kDa was the most important in A. 
      scholaris pollen extract. This component could be purified and would be helpful 
      in the diagnosis and therapy of A. scholaris pollen-susceptible patients.
FAU - Hussain, Mir Musaraf
AU  - Hussain MM
AD  - Department of Botany, Visva-Bharati University, Santiniketan, 731235, West 
      Bengal, India.
FAU - Mandal, Jyotshna
AU  - Mandal J
FAU - Bhattacharya, Kashinath
AU  - Bhattacharya K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130821
PL  - Netherlands
TA  - Environ Monit Assess
JT  - Environmental monitoring and assessment
JID - 8508350
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*analysis/chemistry/immunology
MH  - Alstonia/*physiology
MH  - Atmosphere
MH  - Environmental Monitoring
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoblotting
MH  - Immunoglobulin E/immunology
MH  - India
MH  - Pollen/chemistry/*immunology
MH  - Seasons
MH  - Skin Tests
EDAT- 2013/08/22 06:00
MHDA- 2014/02/28 06:00
CRDT- 2013/08/22 06:00
PHST- 2013/04/12 00:00 [received]
PHST- 2013/08/10 00:00 [accepted]
PHST- 2013/08/22 06:00 [entrez]
PHST- 2013/08/22 06:00 [pubmed]
PHST- 2014/02/28 06:00 [medline]
AID - 10.1007/s10661-013-3390-1 [doi]
PST - ppublish
SO  - Environ Monit Assess. 2014 Jan;186(1):457-67. doi: 10.1007/s10661-013-3390-1. 
      Epub 2013 Aug 21.