PMID- 23966554
OWN - NLM
STAT- MEDLINE
DCOM- 20140421
LR  - 20211021
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 20
IP  - 10
DP  - 2013 Oct
TI  - Identification of a highly antigenic region of subtilisin-like serine protease 1 
      for serodiagnosis of Neospora caninum infection.
PG  - 1617-22
LID - 10.1128/CVI.00352-13 [doi]
AB  - Neospora caninum is an apicomplexan parasite that causes abortion in cattle; 
      hence, accurate diagnosis of this pathogen is important to the cattle farming 
      industry. Our previous proteomics and immunoscreening analyses revealed that the 
      N. caninum subtilisin-like serine protease 1 (NcSUB1) has potential as a 
      serodiagnostic tool for Neospora. Consequently, we expressed two fragments 
      containing five NcSUB1 tandem repeat copies covering amino acids (aa) 524 to 843 
      (NcSUB1t) and 555 to 679 (NcSUB1tr) to identify the antigenic regions. The 
      serodiagnostic performances of NcSUB1t and NcSUB1tr were compared with that of 
      N54, which contains a single copy of the repeats (aa 649 to 784), and with the 
      truncated NcSAG1 (NcSAG1t), which lacks a signal peptide and C-terminal 
      hydrophobic regions, as a positive reference. Serum samples from N. caninum 
      experimentally infected cattle and mice and cattle from a farm with confirmed 
      cases of Neospora abortion were tested by enzyme-linked immunosorbent assay 
      (ELISA) with the four antigens. In the N. caninum experimentally infected cattle, 
      the highest IgG1 antibody titers were detected against NcSUB1t, while specific 
      IgG1 antibodies were detectable from 16 days postinfection (dpi), with levels 
      peaking at 36 dpi for all of the antigens. On the other hand, the levels of 
      anti-NcSUB1 IgG2 antibodies were lower than those of anti-SAG1t IgG2 antibodies. 
      The ELISA with NcSUB1t and NcSUB1tr had good sensitivity (94.59 to 95.95%) and 
      specificity (80 to 100%) with bovine serum field samples compared to NcSAG1t and 
      showed no cross-reactions with sera from Toxoplasma gondii experimentally 
      infected mice. Moreover, IgG antibodies against NcSUB1t were detected during 
      parturition in the NcSAG1t antibody-positive cattle, and NcSUB1t-specific 
      antibody transfer was observed from a mother to her calf. Our results show that 
      the NcSUB1 tandem repeat is potentially useful for serodiagnosis of N. caninum.
FAU - Ybanez, Rochelle Haidee D
AU  - Ybanez RH
AD  - National Research Center for Protozoan Diseases, Obihiro University of 
      Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.
FAU - Terkawi, Mohamad Alaa
AU  - Terkawi MA
FAU - Kameyama, Kyohko
AU  - Kameyama K
FAU - Xuan, Xuenan
AU  - Xuan X
FAU - Nishikawa, Yoshifumi
AU  - Nishikawa Y
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130821
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 3.4.21.- (Proprotein Convertases)
SB  - IM
MH  - Animals
MH  - Antibodies, Protozoan/*blood
MH  - *Antigens, Protozoan/genetics/immunology
MH  - Cattle
MH  - Cattle Diseases/diagnosis
MH  - Coccidiosis/diagnosis/*veterinary
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Female
MH  - Immunoglobulin G/blood
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neospora/*immunology
MH  - *Proprotein Convertases/genetics/immunology
MH  - Recombinant Fusion Proteins/genetics/immunology
MH  - Sensitivity and Specificity
MH  - Serologic Tests/methods
PMC - PMC3807189
EDAT- 2013/08/24 06:00
MHDA- 2014/04/22 06:00
PMCR- 2014/04/01
CRDT- 2013/08/23 06:00
PHST- 2013/08/23 06:00 [entrez]
PHST- 2013/08/24 06:00 [pubmed]
PHST- 2014/04/22 06:00 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - CVI.00352-13 [pii]
AID - 00352-13 [pii]
AID - 10.1128/CVI.00352-13 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2013 Oct;20(10):1617-22. doi: 10.1128/CVI.00352-13. Epub 
      2013 Aug 21.