PMID- 23970374
OWN - NLM
STAT- MEDLINE
DCOM- 20131126
LR  - 20171017
IS  - 1545-5017 (Electronic)
IS  - 1545-5009 (Linking)
VI  - 60
IP  - 12
DP  - 2013 Dec
TI  - Overexpression of miR-708 and its targets in the childhood common precursor 
      B-cell ALL.
PG  - 2060-7
LID - 10.1002/pbc.24583 [doi]
AB  - BACKGROUND: The critical function of microRNAs in the pathogenesis and prognosis 
      of hematopoietic cancer has become increasingly apparent. However, only a few 
      miRNAs have been reported to be altered in acute lymphocytic leukemia (ALL). 
      PROCEDURES: To uncover aberrantly expressed miRNAs in pediatric B-cell ALL, our 
      study employed genome-wide miRNA microarray analysis and stem-loop real-time 
      quantitative polymerase chain reaction (qRT-PCR) to examine common precursor 
      B-cell ALL samples. The target genes of miRNA-708 were then identified and 
      verified by bioinformatics, dual-luciferase reporter assay, qRT-PCR, and Western 
      blot. RESULTS: Significant upregulation of miR-708, miR-210, and miR-181b, and 
      downregulation of miR-345 and miR-27a were observed in common precursor B-cell 
      ALL (common-ALL) samples (P < 0.05). In addition, elevated expression of miR-708 
      and miR-181b were found in high-risk common-ALL compared to standard and 
      intermediate ones. miR-708 inhibited luciferase reporter activity by binding to 
      the 3'-untranslated regions (3'-UTRs) of CNTFR, NNAT, and GNG12 mRNA in HEK-293 
      cell line and suppressed the protein levels of CNTFR, NNAT, and GNG12 in Jurkat 
      cells. In addition, mRNA levels of CNTFR and NNAT, but not of GNG12, were found 
      to be downregulated in high risk common-ALL samples. Mutational analysis revealed 
      that miR-708 binds to the 394-400 bp sequence region of the 3'-UTR of CNTFR mRNA. 
      CONCLUSION: The expression level of miR-708 reflects differences among the 
      clinical types of common-ALL, and CNTFR, NNAT, and GNG12 were identified as 
      targets of miR-708.
CI  - (c) 2013 Wiley Periodicals, Inc.
FAU - Li, Xue
AU  - Li X
AD  - Department of Pediatrics, Cryomedicine Laboratory, Qilu Hospital, Shandong 
      University, Shandong, PR China.
FAU - Li, Dong
AU  - Li D
FAU - Zhuang, Yong
AU  - Zhuang Y
FAU - Shi, Qing
AU  - Shi Q
FAU - Wei, Wei
AU  - Wei W
FAU - Ju, Xiuli
AU  - Ju X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Retracted Publication
DEP - 20130823
PL  - United States
TA  - Pediatr Blood Cancer
JT  - Pediatric blood & cancer
JID - 101186624
RN  - 0 (CNTFR protein, human)
RN  - 0 (Ciliary Neurotrophic Factor Receptor alpha Subunit)
RN  - 0 (GNG2 protein, human)
RN  - 0 (MIRN708 microRNA, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (MicroRNAs)
RN  - 0 (NNAT protein, human)
RN  - 0 (Nerve Tissue Proteins)
RN  - EC 3.6.1.- (GTP-Binding Proteins)
SB  - IM
CIN - Pediatr Blood Cancer. 2015 Jan;62(1):177-8. PMID: 25214155
RIN - Pediatr Blood Cancer. 2017 May;64(5):null. PMID: 28342301
MH  - Blotting, Western
MH  - Child
MH  - Child, Preschool
MH  - Ciliary Neurotrophic Factor Receptor alpha Subunit/biosynthesis/genetics
MH  - Female
MH  - GTP-Binding Proteins/biosynthesis/genetics
MH  - Gene Expression Regulation, Neoplastic/*genetics
MH  - Humans
MH  - Infant
MH  - Male
MH  - Membrane Proteins/biosynthesis/genetics
MH  - MicroRNAs/*biosynthesis/genetics
MH  - Microarray Analysis
MH  - Nerve Tissue Proteins/biosynthesis/genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Up-Regulation
OTO - NOTNLM
OT  - miRNA
OT  - pediatric acute lymphoblastic leukemia
OT  - regulatory region
OT  - target gene
EDAT- 2013/08/24 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/08/24 06:00
PHST- 2013/01/08 00:00 [received]
PHST- 2013/04/07 00:00 [accepted]
PHST- 2013/08/24 06:00 [entrez]
PHST- 2013/08/24 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 10.1002/pbc.24583 [doi]
PST - ppublish
SO  - Pediatr Blood Cancer. 2013 Dec;60(12):2060-7. doi: 10.1002/pbc.24583. Epub 2013 
      Aug 23.