PMID- 23972455
OWN - NLM
STAT- MEDLINE
DCOM- 20140224
LR  - 20191210
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1309
DP  - 2013 Sep 27
TI  - Determination of selected perfluorinated alkyl acids and persistent organic 
      pollutants from a small volume human serum sample relevant for epidemiological 
      studies.
PG  - 48-55
LID - S0021-9673(13)01124-2 [pii]
LID - 10.1016/j.chroma.2013.07.064 [doi]
AB  - A simple and fast method is presented for the determination of selected 
      persistent organic pollutants (POPs) and perfluorinated alkyl acids (PFAAs), a 
      subgroup of per- and polyfluorinated alkyl substances (PFAS) from a single 200mul 
      aliquot of serum. Sample pretreatment starts with dispersive solid phase 
      extraction of POPs to dichloromethane-hexane, which is immediately poured to 
      cleanup column. POPs are eluted from column and concentrated for GC-MS/MS 
      analysis. PFAAs are trapped to dispersant and are then extracted with ammonium 
      acetate in methanol, concentrated and analysed with LC-MS/MS. For POPs, the limit 
      of detection (LOD) ranged from 1.6 to 17pg/ml. Oxychlordane and 
      dichlorodiphenyltrichloroethane had LODs in the upper end of this range as they 
      were more labile and prone for interferences in the GC-MS/MS. For PFAAs, the LOD 
      range from 0.027 to 0.068ng/ml. For POPs, the accuracy from Standard Reference 
      Materials SRM 1589a and Arctic Monitoring and Assessment Programme (AMAP) 
      intercalibration samples range from 74 to 127% and the repeatability (relative 
      standard deviation, RSD%) from 2.0 to 15%. For PFAAs, the accuracy from AMAP 
      samples ranged from 90 to 110% and from LOQ level spiked serum samples from 72 to 
      133%. Repeatability from AMAP and LOQ samples ranged from 1.6 to 7.3% and 5.5 to 
      15%, respectively. The presented method is useful in epidemiological studies 
      where only limited amount of serum is available.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Koponen, Jani
AU  - Koponen J
AD  - National Institute for Health and Welfare, Department of Environmental Health, 
      P.O. Box 95, FI-70701 Kuopio, Finland. ani.koponen@thl.fi
FAU - Rantakokko, Panu
AU  - Rantakokko P
FAU - Airaksinen, Riikka
AU  - Airaksinen R
FAU - Kiviranta, Hannu
AU  - Kiviranta H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20130719
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Environmental Pollutants)
RN  - 0 (Fluorocarbons)
RN  - 0 (Organic Chemicals)
RN  - 76-21-1 (hexadecafluoro-nonanoic acid)
SB  - IM
MH  - Environmental Exposure/analysis
MH  - Environmental Pollutants/*blood/isolation & purification
MH  - Epidemiologic Studies
MH  - Fluorocarbons/*blood/isolation & purification
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Humans
MH  - Limit of Detection
MH  - Organic Chemicals/*blood/isolation & purification
MH  - Solid Phase Extraction
OTO - NOTNLM
OT  - Cardiovascular disease
OT  - Dispersive solid phase extraction
OT  - Human serum
OT  - Perfluorinated alkyl substances
OT  - Persistent organic pollutants
OT  - Type 2 diabetes
EDAT- 2013/08/27 06:00
MHDA- 2014/02/25 06:00
CRDT- 2013/08/27 06:00
PHST- 2013/04/22 00:00 [received]
PHST- 2013/07/12 00:00 [revised]
PHST- 2013/07/15 00:00 [accepted]
PHST- 2013/08/27 06:00 [entrez]
PHST- 2013/08/27 06:00 [pubmed]
PHST- 2014/02/25 06:00 [medline]
AID - S0021-9673(13)01124-2 [pii]
AID - 10.1016/j.chroma.2013.07.064 [doi]
PST - ppublish
SO  - J Chromatogr A. 2013 Sep 27;1309:48-55. doi: 10.1016/j.chroma.2013.07.064. Epub 
      2013 Jul 19.