PMID- 23973786
OWN - NLM
STAT- MEDLINE
DCOM- 20140519
LR  - 20131011
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 150
IP  - 1
DP  - 2013 Oct 28
TI  - Neuroprotective effects of Polygonum multiflorum extract against 
      glutamate-induced oxidative toxicity in HT22 hippocampal cells.
PG  - 108-15
LID - S0378-8741(13)00556-4 [pii]
LID - 10.1016/j.jep.2013.08.014 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Dried roots of Polygonum multiflorum have 
      traditionally been used in the retarding of aging process in East Asian countries 
      and its extracts exhibit anti-oxidative activities. MATERIALS AND METHODS: 
      Neuroprotective effects of ethyl acetate extract from Polygonum multiflorum 
      (EEPM) were investigated against glutamate-induced oxidative cell death in HT22 
      hippocampal cells. Cell viability, cytotoxicity, morphological, flow cytometry, 
      and Western blot assays were performed in order to observe alterations of 
      neuronal cell survival or death related pathways. RESULTS: Pretreatment with EEPM 
      resulted in significantly decreased glutamate-induced neurotoxicity and also 
      resulted in drastically inhibited glutamate-induced apoptotic and necrotic 
      neuronal death. To elucidate possible pathways of neuroprotection by EEPM, we 
      explored the activation of mitogen activated protein kinases (MAPKs), 
      phosphatidylinositol-3-kinase, and cAMP responsive element binding protein 
      (CREB). Treatment with glutamate alone led to activation of extracellular 
      regulated kinase (ERK), Jun N-terminal kinase, and p38 during the late phase 
      after glutamate exposure, but pretreatment with EEPM resulted in significantly 
      attenuated activation of these proteins. Pretreatment with EEPM resulted in 
      increased activation of CREB. The specific inhibitors of ERK and p38, PD98059 and 
      SB203580, abrogated the neuroprotective effects of EEPM. When we evaluated 
      calpain I and striatal-enriched protein tyrosine phosphatase (STEP), active form 
      of calpain I was significantly increased after glutamate exposure, and, along 
      with this, active form of STEP showed a decrease. Pretreatment with EEPM resulted 
      in significant recovery of pro-calpain I and active form of STEP caused by 
      glutamate. Co-treatment with calpain inhibitor ALLN and EEPM had a synergistic 
      effect on neuronal death and contributed to blockade of activation of both ERK 
      and p38 with increased activation of CREB. CONCLUSIONS: These results suggest 
      that Polygonum multiflorum extract may have neuroprotective effects through both 
      alleviation of ERK and p38 activation with increased activation of CREB under 
      oxidative stress and has potential as a therapeutic intervention for treatment of 
      oxidative neuronal death.
CI  - (c) 2013 Elsevier Ireland Ltd. All rights reserved.
FAU - Kim, Ha Neui
AU  - Kim HN
AD  - Department of Korean Medical Science, School of Korean Medicine, Pusan National 
      University, Yangsan 626-870, Republic of Korea.
FAU - Kim, Yu Ri
AU  - Kim YR
FAU - Jang, Ji Yeon
AU  - Jang JY
FAU - Choi, Young Whan
AU  - Choi YW
FAU - Baek, Jin Ung
AU  - Baek JU
FAU - Hong, Jin Woo
AU  - Hong JW
FAU - Choi, Yung Hyun
AU  - Choi YH
FAU - Shin, Hwa Kyoung
AU  - Shin HK
FAU - Choi, Byung Tae
AU  - Choi BT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130822
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Creb1 protein, mouse)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Plant Extracts)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.4.22.- (Calpain)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Calpain/metabolism
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Glutamic Acid
MH  - Hippocampus/cytology
MH  - Mice
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Neuroprotective Agents/*pharmacology
MH  - Oxidative Stress/drug effects
MH  - Plant Extracts/*pharmacology
MH  - Plant Roots
MH  - *Polygonum
OTO - NOTNLM
OT  - ERK
OT  - HT22 cell
OT  - Oxidative neurotoxicity
OT  - Polygonum multiflorum
OT  - p38
EDAT- 2013/08/27 06:00
MHDA- 2014/05/20 06:00
CRDT- 2013/08/27 06:00
PHST- 2013/04/12 00:00 [received]
PHST- 2013/07/12 00:00 [revised]
PHST- 2013/08/11 00:00 [accepted]
PHST- 2013/08/27 06:00 [entrez]
PHST- 2013/08/27 06:00 [pubmed]
PHST- 2014/05/20 06:00 [medline]
AID - S0378-8741(13)00556-4 [pii]
AID - 10.1016/j.jep.2013.08.014 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2013 Oct 28;150(1):108-15. doi: 10.1016/j.jep.2013.08.014. Epub 
      2013 Aug 22.