PMID- 23973796 OWN - NLM STAT- MEDLINE DCOM- 20140801 LR - 20180816 IS - 1872-8111 (Electronic) IS - 0168-0102 (Linking) VI - 77 IP - 4 DP - 2013 Dec TI - DNA methylation analysis of BDNF gene promoters in peripheral blood cells of schizophrenia patients. PG - 208-14 LID - S0168-0102(13)00189-2 [pii] LID - 10.1016/j.neures.2013.08.004 [doi] AB - Accumulating evidence suggests that epigenetic alterations in brain-derived neurotrophic factor (BDNF) promoters are associated with the pathophysiology of psychiatric disorders. Epigenetic changes in BDNF were reported not only in brain tissues but also in other tissues, including peripheral blood cells (PBC) and saliva. We examined DNA methylation levels of BDNF promoters I and IV using genomic DNA derived from PBC of healthy controls (n=100), and patients with schizophrenia (n=100), all from the Japanese population, by pyrosequencing. The examined CpG sites were chosen based on previous epigenetic studies that reported altered DNA methylation. We found a significantly higher level of methylation at BDNF promoter I in patients with schizophrenia compared to controls, although the difference was small. Subsequent analysis revealed that in controls, the methylation level of BDNF promoters was associated with sex, and the methylation difference observed in promoter I was more prominent in male patients with schizophrenia. Epigenetic alteration of BDNF in the PBC might reflect the pathophysiology of schizophrenia, and could be a potential biomarker. CI - Copyright (c) 2013 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved. FAU - Ikegame, Tempei AU - Ikegame T AD - Department of Molecular Psychiatry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan; Department of Neuropsychiatry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan. FAU - Bundo, Miki AU - Bundo M FAU - Sunaga, Fumiko AU - Sunaga F FAU - Asai, Tatsuro AU - Asai T FAU - Nishimura, Fumichika AU - Nishimura F FAU - Yoshikawa, Akane AU - Yoshikawa A FAU - Kawamura, Yoshiya AU - Kawamura Y FAU - Hibino, Hiroyuki AU - Hibino H FAU - Tochigi, Mamoru AU - Tochigi M FAU - Kakiuchi, Chihiro AU - Kakiuchi C FAU - Sasaki, Tsukasa AU - Sasaki T FAU - Kato, Tadafumi AU - Kato T FAU - Kasai, Kiyoto AU - Kasai K FAU - Iwamoto, Kazuya AU - Iwamoto K LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130822 PL - Ireland TA - Neurosci Res JT - Neuroscience research JID - 8500749 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 7171WSG8A2 (BDNF protein, human) SB - IM MH - Adult MH - Age Factors MH - Brain-Derived Neurotrophic Factor/blood/*genetics MH - *DNA Methylation MH - Female MH - Humans MH - Male MH - Middle Aged MH - *Promoter Regions, Genetic MH - Schizophrenia/blood/*genetics MH - Sex Factors OTO - NOTNLM OT - Epigenetic OT - Gender difference OT - Peripheral blood OT - Pyrosequencing EDAT- 2013/08/27 06:00 MHDA- 2014/08/02 06:00 CRDT- 2013/08/27 06:00 PHST- 2013/07/08 00:00 [received] PHST- 2013/08/08 00:00 [revised] PHST- 2013/08/12 00:00 [accepted] PHST- 2013/08/27 06:00 [entrez] PHST- 2013/08/27 06:00 [pubmed] PHST- 2014/08/02 06:00 [medline] AID - S0168-0102(13)00189-2 [pii] AID - 10.1016/j.neures.2013.08.004 [doi] PST - ppublish SO - Neurosci Res. 2013 Dec;77(4):208-14. doi: 10.1016/j.neures.2013.08.004. Epub 2013 Aug 22.