PMID- 23974966
OWN - NLM
STAT- MEDLINE
DCOM- 20141011
LR  - 20211203
IS  - 1432-2013 (Electronic)
IS  - 0031-6768 (Print)
IS  - 0031-6768 (Linking)
VI  - 466
IP  - 3
DP  - 2014 Mar
TI  - Ambient hypoxia enhances the loss of muscle mass after extensive injury.
PG  - 587-98
LID - 10.1007/s00424-013-1336-7 [doi]
AB  - Hypoxia induces a loss of skeletal muscle mass and alters myogenesis in vitro, 
      but whether it affects muscle regeneration in vivo following injury remains to be 
      elucidated. We hypothesized that hypoxia would impair the recovery of muscle mass 
      during regeneration. To test this hypothesis, the soleus muscle of female rats 
      was injured by notexin and allowed to recover for 3, 7, 14, and 28 days under 
      normoxia or hypobaric hypoxia (5,500 m) conditions. Hypoxia impaired the 
      formation and growth of new myofibers and enhanced the loss of muscle mass during 
      the first 7 days of regeneration, but did not affect the final recovery of muscle 
      mass at 28 days. The impaired regeneration under hypoxic conditions was 
      associated with a blunted activation of mechanical target of rapamycin (mTOR) 
      signaling as assessed by p70(S6K) and 4E-BP1 phosphorylation that was independent 
      of Akt activation. The decrease in mTOR activity with hypoxia was consistent with 
      the increase in AMP-activated protein kinase activity, but not related to the 
      change in regulated in development and DNA response 1 protein content. Hypoxia 
      increased the mRNA levels of the atrogene muscle ring finger-1 after 7 days of 
      regeneration, though muscle atrophy F box transcript levels remained unchanged. 
      The increase in MyoD and myogenin mRNA expression with regeneration was 
      attenuated at 7 days with hypoxia. In conclusion, our results support the notion 
      that the enhanced loss of muscle mass observed after 1 week of regeneration under 
      hypoxic conditions could mainly result from the impaired formation and growth of 
      new fibers resulting from a reduction in protein synthesis and satellite cell 
      activity.
FAU - Chaillou, T
AU  - Chaillou T
AD  - Departement Environnements operationnels, Institut de Recherche Biomedicale des 
      Armees, antenne de La Tronche, BP87, 38702, La Tronche, France, 
      th.chaillou@gmail.com.
FAU - Koulmann, N
AU  - Koulmann N
FAU - Meunier, A
AU  - Meunier A
FAU - Pugniere, P
AU  - Pugniere P
FAU - McCarthy, J J
AU  - McCarthy JJ
FAU - Beaudry, M
AU  - Beaudry M
FAU - Bigard, X
AU  - Bigard X
LA  - eng
GR  - R01 AR061939/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130824
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
RN  - 0 (Carrier Proteins)
RN  - 0 (Ddit4 protein, rat)
RN  - 0 (Eif4ebp1 protein, rat)
RN  - 0 (Elapid Venoms)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (MyoD Protein)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Tripartite Motif Proteins)
RN  - 37223-96-4 (notexin)
RN  - EC 2.3.2.27 (Trim63 protein, rat)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Animals
MH  - Carrier Proteins/metabolism
MH  - Cell Hypoxia
MH  - Elapid Venoms/toxicity
MH  - Female
MH  - Hypoxia/*metabolism/physiopathology
MH  - Intracellular Signaling Peptides and Proteins
MH  - Muscle Fibers, Skeletal/drug effects/*metabolism/pathology/physiology
MH  - Muscle Proteins/genetics/metabolism
MH  - MyoD Protein/genetics/metabolism
MH  - Phosphoproteins/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - *Regeneration
MH  - Repressor Proteins/genetics/metabolism
MH  - Ribosomal Protein S6 Kinases/metabolism
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Transcription Factors
MH  - Tripartite Motif Proteins
MH  - Ubiquitin-Protein Ligases/genetics/metabolism
PMC - PMC4878136
MID - NIHMS785892
EDAT- 2013/08/27 06:00
MHDA- 2014/10/12 06:00
PMCR- 2016/05/24
CRDT- 2013/08/27 06:00
PHST- 2013/05/09 00:00 [received]
PHST- 2013/08/10 00:00 [accepted]
PHST- 2013/08/09 00:00 [revised]
PHST- 2013/08/27 06:00 [entrez]
PHST- 2013/08/27 06:00 [pubmed]
PHST- 2014/10/12 06:00 [medline]
PHST- 2016/05/24 00:00 [pmc-release]
AID - 10.1007/s00424-013-1336-7 [doi]
PST - ppublish
SO  - Pflugers Arch. 2014 Mar;466(3):587-98. doi: 10.1007/s00424-013-1336-7. Epub 2013 
      Aug 24.