PMID- 2397564
OWN - NLM
STAT- MEDLINE
DCOM- 19901018
LR  - 20131121
IS  - 0263-6484 (Print)
IS  - 0263-6484 (Linking)
VI  - 8
IP  - 3
DP  - 1990 Jul
TI  - ATP modifications in hexokinase deficient fibroblasts exposed to nutrient shifts.
PG  - 167-70
AB  - Human fibroblasts from a patient homozygous for hexokinase (HK) deficiency and 
      normal controls were submitted to different nutrient shifts to investigate the 
      role of glucose metabolism on ATP levels. HK-deficient cells maintained in the 
      presence of both glucose and L-glutamine had 20 per cent less ATP than controls 
      and an ATP/ADP ratio of 18 instead of 37-40. Glucose alone was not able to 
      maintain normal ATP levels neither in the controls nor in the HK-deficient cells. 
      However while in control cells the ATP/ADP ratio was not modified, it was reduced 
      to 10 in HK deficiency. The effect of several hexoses on ATP levels was also 
      investigated. Mannose, which is metabolized by HK phosphorylation, was the least 
      efficient for ATP maintenance in HK-deficient cells. In contrast galactose, which 
      is metabolized by a specific galactokinase, provided ATP values close to those 
      observed in the presence of glutamine. These results suggest that glucose 
      metabolism is an important determinant in the maintenance of ATP levels in 
      cultured cells and that glutamine, although being a good metabolic substrate, is 
      not sufficient to maintain normal ATP concentrations.
FAU - Chiarantini, L
AU  - Chiarantini L
AD  - Istituto di Chimica Biologica G. Fornaini, Universita degli Studi, Urbino, Italy.
FAU - Vittoria, E
AU  - Vittoria E
FAU - Magnani, M
AU  - Magnani M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
RN  - 0RH81L854J (Glutamine)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adenosine Diphosphate/metabolism
MH  - Adenosine Triphosphate/*metabolism
MH  - Adult
MH  - Cell Line
MH  - Fibroblasts/metabolism
MH  - Glucose/metabolism
MH  - Glutamine/metabolism
MH  - Hexokinase/*deficiency
MH  - Humans
MH  - Nutritional Status
EDAT- 1990/07/01 00:00
MHDA- 1990/07/01 00:01
CRDT- 1990/07/01 00:00
PHST- 1990/07/01 00:00 [pubmed]
PHST- 1990/07/01 00:01 [medline]
PHST- 1990/07/01 00:00 [entrez]
AID - 10.1002/cbf.290080307 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 1990 Jul;8(3):167-70. doi: 10.1002/cbf.290080307.