PMID- 23976822
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211021
IS  - 0934-0866 (Print)
IS  - 1521-4117 (Electronic)
IS  - 0934-0866 (Linking)
VI  - 30
IP  - 4
DP  - 2013 Apr
TI  - Anti-CRLF2 Antibody-Armored Biodegradable Nanoparticles for Childhood B-ALL.
PG  - 355-364
AB  - B-precursor acute lymphoblastic leukemia (B-ALL) lymphoblast (blast) 
      internalization of anti-cytokine receptor-like factor 2 (CRLF2) antibody-armored 
      biodegradable nanoparticles (AbBNPs) are investigated. First, AbBNPsaere 
      synthesized by adsorbing anti-CRLF2 antibodies to poly(D,L-lactide- co 
      -glycolide) (PLGA) nanoparticles of various sizes and antibody surface density 
      (Ab/BNP) ratios. Second, AbBNPs are incubated with CRLF2-overexpressing (CRLF2+) 
      or control blasts. Third, internalization of AbBNPs by blasts is evaluated by 
      multicolor flow cytometry as a function of receptor expression, AbBNP size, and 
      Ab/BNP ratio. Results from these experiments are con-firmed by electron 
      microscopy, fluorescence microscopy, and Western blotting. The optimal size and 
      Ab/BNP for internalization of AbBNPs by CRLF2+ blasts is 50 nm with 10 Ab/BNP and 
      100 nm with 25 Ab/BNP. These studies show that internalization of AbBNPs in 
      childhood B-ALL blasts is AbBNP size-and Ab/BNP ratio-dependent. All AbBNP 
      combinations are non-cytotoxic. It is also shown that CD47 is very slightly 
      up-regulated by blasts exposed to AbBNPs. CD47 is "the marker of self" 
      overexpressed by blasts to escape phagocytosis, or "cellular devouring", by 
      beneficial macrophages. The results indicate that precise engineering of AbBNPs 
      by size and Ab/BNP ratio may improve the internalization and selectivity of 
      future biodegradable nanoparticles for the treatment of leukemia patients, 
      including drug-resistant minority children and Down's syndrome patients with 
      CRLF2+B-ALL.
FAU - Raghunathan, Rekha
AU  - Raghunathan R
AD  - Pediatric Biochemistry Laboratory Department of Chemistry-BSE 3.108A University 
      of Texas at San Antonio One UTSA Circle, San Antonio, TX 78249, USA.
FAU - Mahesula, Swetha
AU  - Mahesula S
FAU - Kancharla, Kranthi
AU  - Kancharla K
FAU - Janardhanan, Preethi
AU  - Janardhanan P
FAU - Jadhav, Yeshwant L A
AU  - Jadhav YL
FAU - Nadeau, Robert
AU  - Nadeau R
FAU - Villa, German P
AU  - Villa GP
FAU - Cook, Robert L
AU  - Cook RL
FAU - Witt, Colleen M
AU  - Witt CM
FAU - Gelfond, Jonathan A L
AU  - Gelfond JA
FAU - Forsthuber, Thomas G
AU  - Forsthuber TG
FAU - Haskins, William E
AU  - Haskins WE
LA  - eng
GR  - U54 RR022762/RR/NCRR NIH HHS/United States
GR  - L40 CA130418/CA/NCI NIH HHS/United States
GR  - P30 CA054174/CA/NCI NIH HHS/United States
GR  - U54 MD008149/MD/NIMHD NIH HHS/United States
GR  - G12 MD007591/MD/NIMHD NIH HHS/United States
GR  - G12 RR013646/RR/NCRR NIH HHS/United States
PT  - Journal Article
PL  - Germany
TA  - Part Part Syst Charact
JT  - Particle & particle systems characterization : measurement and description of 
      particle properties and behavior in powders and other disperse systems
JID - 9891749
PMC - PMC3746557
MID - NIHMS481324
EDAT- 2013/08/27 06:00
MHDA- 2013/08/27 06:01
PMCR- 2014/04/01
CRDT- 2013/08/27 06:00
PHST- 2013/08/27 06:00 [entrez]
PHST- 2013/08/27 06:00 [pubmed]
PHST- 2013/08/27 06:01 [medline]
PHST- 2014/04/01 00:00 [pmc-release]
AID - 10.1002/ppsc.201200125 [doi]
PST - ppublish
SO  - Part Part Syst Charact. 2013 Apr;30(4):355-364. doi: 10.1002/ppsc.201200125.