PMID- 23978018
OWN - NLM
STAT- MEDLINE
DCOM- 20140515
LR  - 20171116
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 34
IP  - 8
DP  - 2013 Aug
TI  - [Effects of p38MAPK inhibitor on the occurrence of acute GVHD and intestine 
      damage after allogeneic hematopoietic stem cell transplantation in mice].
PG  - 673-8
LID - 10.3760/cma.j.issn.0253-2727.2013.08.007 [doi]
AB  - OBJECTIVE: To explore the effects of p38MAPK inhibitor SB203580 (SB) on the 
      occurrence of acute GVHD and intestine damage after allogeneic hematopoietic stem 
      cell transplantation (allo-HSCT) in mice. METHODS: Sixty BALB/c mice, as 
      recipients, were randomized to control group, irradiation group, model group and 
      intervention group. C57BL/6 mice, as donors, were raised to prepare the bone 
      marrow cells (BMCs) and spleen cells (SCs), which were injected into irradiated 
      recipients mice by tail vein. Except control group, other groups accepted 7.5Gy 
      total body irradiation. Model group and intervention group were infused with BMCs 
      5x10(6) and SCs 5x10(5) by less than 4 h after irradiation. SB was injected into 
      intervention group by intraperitoneally, but only DMSO for model group. The 
      general status and survival rate of each group were evaluated. The expression of 
      p-p38MAPK, Fas and FasL in intestine were determined by RT-PCR, Western blot and 
      immunohistochemistry (IHC). RESULTS: The weight changes of intervention group 
      (13.00+/-0.50)% was significantly lighter than that of model group (25.00+/-0.75)% 
      (P<0.05). The clinical score of acute GVHD in the intervention group (3.33+/-0.82) 
      was significantly lower than that of model group (6.33+/-1.36) (P<0.05). The 
      expression levels of p-p38MAPK, Fas and FasL in small intestine of intervention 
      group (1.43+/-0.02, 0.81+/-0.03, 0.97+/-0.03) were lower than those of model group 
      (1.76+/-0.05, 1.52+/-0.04, 1.48+/-0.04). CONCLUSION: SB inhibited the activation of 
      p38MAPK and Fas/ FasL signal pathway and alleviated the apoptosis of small 
      intestine. And SB could relieve small intestine damages induced by allogeneic T 
      lymphocytes.
FAU - Zhang, Cui-ping
AU  - Zhang CP
AD  - Department of Pathogenic Biology and Immunology, Xuzhou Medical College, Xuzhou 
      221002, China.
FAU - Li, Xiao-cui
AU  - Li XC
FAU - Tang, Ren-xian
AU  - Tang RX
FAU - Li, Xiang-yang
AU  - Li XY
FAU - Zheng, Kui-yang
AU  - Zheng KY
FAU - Zeng, Ling-yu
AU  - Zeng LY
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Fas protein, mouse)
RN  - 0 (Fasl protein, mouse)
RN  - 0 (Imidazoles)
RN  - 0 (Pyridines)
RN  - 0 (fas Receptor)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - OU13V1EYWQ (SB 203580)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Bone Marrow Transplantation/adverse effects
MH  - Fas Ligand Protein/metabolism
MH  - Graft vs Host Disease/metabolism/*pathology
MH  - Imidazoles/*pharmacology
MH  - Intestines/*drug effects/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Pyridines/*pharmacology
MH  - Signal Transduction/drug effects
MH  - Transplantation, Homologous
MH  - fas Receptor/metabolism
MH  - p38 Mitogen-Activated Protein Kinases/*antagonists & inhibitors/metabolism
EDAT- 2013/08/28 06:00
MHDA- 2014/05/16 06:00
CRDT- 2013/08/28 06:00
PHST- 2013/08/28 06:00 [entrez]
PHST- 2013/08/28 06:00 [pubmed]
PHST- 2014/05/16 06:00 [medline]
AID - 10.3760/cma.j.issn.0253-2727.2013.08.007 [doi]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2013 Aug;34(8):673-8. doi: 
      10.3760/cma.j.issn.0253-2727.2013.08.007.